Hprt1<tm335(Ple277-icre/ERT2)Ems> Targeted Allele Detail MGI Mouse (MGI:5532381)

Hprt1^{tm335(Ple277-icre/ERT2)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm335(Ple277-icre/ERT2)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 335, Elizabeth M Simpson
MGI ID:	MGI:5532381
Synonyms:	HTR1B-creERT2, Ple277-icre/ERT2;mEMS5794
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm335(Ple277-icre/ERT2)Ems} page

Mutation
origin

Mutant Cell Line:	mEMS5794
Germline Transmission:	Earliest citation of germline transmission: J:204202
Parent Cell Line:	mEMS4857.02 (ES Cell)
Strain of Origin:	B6N.Cg-A^w-J Hprt1^b-m3
Project Collection:	EUCOMMTOOLS

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Hprt1^{tm335(Ple277-icre/ERT2)Ems} expression driven by 1 gene

Mutation details: The transgene consists of a RP11-990.K4 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and a 48-bp full Frt (Ffrt) site-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human HTR1B locus. Transient transfection with FLP recombinase removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus. (J:270567)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	HTR1B (human) Summary of all recombinase alleles driven by HTR1B. Alliance human HTR1B gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1279 strains or lines available

References

Original:	J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014;
All:	2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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