Hprt1tm339a(Ple280-icre/ERT2)Ems
Targeted Allele Detail
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| Symbol: |
Hprt1tm339a(Ple280-icre/ERT2)Ems |
| Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 339a, Elizabeth M Simpson |
| MGI ID: |
MGI:5532385 |
| Synonyms: |
MKI67-creERT2, Ple280-icre/ERT2;mEMS5857 |
| Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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| Alliance: |
Hprt1tm339a(Ple280-icre/ERT2)Ems page
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| Mutant Cell Line: |
mEMS5857 |
| Germline Transmission: |
Earliest citation of germline transmission:
J:204202
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| Parent Cell Line: |
mEMS4857.02 (ES Cell)
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| Strain of Origin: |
B6N.Cg-Aw-J Hprt1b-m3
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| Project Collection: |
EUCOMMTOOLS
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| Allele Type: |
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Targeted (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Hprt1tm339a(Ple280-icre/ERT2)Ems expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
MKI67 (4288) |
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Mutation details: The transgene consists of a RP11-636.J22 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene, an intron, an SV40 early polyA signal and a 48-bp full Frt (Ffrt) site-flanked kanamycin resistance (Kan) cassette were inserted directly at the ATG of the human MKI67 locus. Transient transfection with a FLP recombinase plasmid removed the Kan cassette before the modified BAC was used for targeting as a single copy "knock-in" into the upstream region of the Hprt locus.
(J:204202)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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MKI67
(human)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hprt1 Mutation: |
1279 strains or lines available
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| Original: |
J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014; |
| All: |
1 reference(s) |
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Analysis Tools
