Hprt1<tm345a(icre/ERT2)Ems> Targeted Allele Detail MGI Mouse (MGI:5532493)

Hprt1^{tm345a(icre/ERT2)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm345a(icre/ERT2)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 345a, Elizabeth M Simpson
MGI ID:	MGI:5532493
Synonyms:	Promoterless-creERT2, Promoterless-icre/frt/ERT2/frt;mEMS5895
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm345a(icre/ERT2)Ems} page

Mutation
origin

Mutant Cell Line:	mEMS5895
Germline Transmission:	Earliest citation of germline transmission: J:204202
Parent Cell Line:	mEMS4855.02 (ES Cell)
Strain of Origin:	B6N.Cg-A^w-J Hprt1^b-m3
Project Collection:	EUCOMMTOOLS

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Hprt1^{tm345a(icre/ERT2)Ems} expression driven by 1 gene

Mutation details: The transgene was designed with an HS4 insulator region, no minipromoter, a chimeric intron cassette, the iCre/ERT2 fusion gene (with F3-frt sites flanking the ERT2 sequences), the mut6-WPRE element, an SV40 early polyA signal, an additional HS4 insulator region, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. The iCre/ERT2 fusion gene used here has an optimized variant of Cre recombinase fused to ERT2; additionally modified to have F3-frt sites flanking the estrogen receptor sequences. (J:270567)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hprt1 (mouse) Summary of all recombinase alleles driven by Hprt1. Alliance mouse Hprt1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1279 strains or lines available

References

Original:	J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014;
All:	3 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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