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Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: RNA in situ
MGI Accession ID: MGI:5540723
Gene symbol: Sema5a
Gene name: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A
Probe: RIKEN clone E030043B19 subclone
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen GUDMAP:9446: embryonic day 10.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS17: genitourinary system Present Regionally restricted GUDMAP:9446
TS17: genitourinary system mesenchyme Present GUDMAP:9446
TS17: mesonephros Present Regionally restricted GUDMAP:9446
TS17: nephric duct, mesonephric portion Present GUDMAP:9446
TS17: metanephric mesenchyme Present GUDMAP:9446 possible regional expression pattern
TS17: nephric duct Present Regionally restricted GUDMAP:9446 graded expression; regional expression with possible gradient of signal seen - weakest in cranial duct and absent from metanephric portion
TS17: nephric duct, metanephric portion Ambiguous GUDMAP:9446 expression is likely to be absent
TS17: mesonephros mesenchyme Ambiguous GUDMAP:9446 uncertain expression
TS17: mesonephric tubule Ambiguous GUDMAP:9446 possible weak expression
Specimen GUDMAP:9447: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: male reproductive system Present Regionally restricted GUDMAP:9447
TS21: mesonephros of male Present Regionally restricted GUDMAP:9447
TS21: mesonephric mesenchyme of male Present Regionally restricted GUDMAP:9447 lateral expression; expression absent from the medial mesenchyme adjacent to the testis; regional expression surrounding the nephric and paramesonephric duct epithelia both in the mesonephros and extending caudally beyond the testis
TS21: nephric duct of male, rest of Present Regionally restricted GUDMAP:9447
TS21: mesenchyme of nephric duct of male Present GUDMAP:9447
TS21: paramesonephric duct of male, rest of Present Regionally restricted GUDMAP:9447
TS21: mesenchyme of rest of paramesonephric duct of male Present GUDMAP:9447
TS21: testis Present GUDMAP:9447 weak signal in the primary sex cords
TS21: primary sex cord Present GUDMAP:9447
TS21: coelomic epithelium of male mesonephros Absent GUDMAP:9447
TS21: epithelium of nephric duct of male Absent GUDMAP:9447
TS21: epithelium of rest of paramesonephric duct of male Absent GUDMAP:9447
TS21: mesonephric tubule of male Ambiguous GUDMAP:9447 expression may be restricted to the mesenchyme surrounding the tubules
TS21: interstitium of the testis Ambiguous GUDMAP:9447 possible expression
TS21: nephric duct of male, mesonephric portion Ambiguous GUDMAP:9447 expression is likely to be absent but the duct is obstructed by the signal in the mesenchyme
TS21: paramesonephric duct of male, mesonephric portion Ambiguous GUDMAP:9447
Specimen GUDMAP:9448: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: urinary system Present GUDMAP:9448
TS21: metanephros Present Regionally restricted GUDMAP:9448
TS21: renal interstitium Present Regionally restricted GUDMAP:9448 signal pattern indicative of regional expression surrounding ureteric trunk; absent from the rest of the interstitium
TS21: renal pelvis Present GUDMAP:9448
TS21: ureter Present GUDMAP:9448 signal may be regional to the presumptive ureteral smooth muscle layer
TS21: ureteral mesenchyme Present GUDMAP:9448
TS21: ureteral mesenchyme middle layer Present GUDMAP:9448
TS21: nephrogenic zone Ambiguous GUDMAP:9448 signal appears to be absent
TS21: early nephron Ambiguous GUDMAP:9448 possible expression
Specimen GUDMAP:9449: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: urinary system Present GUDMAP:9449
TS21: ureter Present Regionally restricted GUDMAP:9449 signal may be regional to the presumptive ureteral smooth muscle layer
TS21: ureteral mesenchyme Present GUDMAP:9449
TS21: ureteral mesenchyme middle layer Present GUDMAP:9449
TS21: urogenital sinus Present GUDMAP:9449
TS21: primitive bladder Present Regionally restricted GUDMAP:9449
TS21: mesenchymal layer of primitive bladder Present GUDMAP:9449
TS21: urothelium of primitive bladder Ambiguous GUDMAP:9449
Specimen GUDMAP:9450: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: female reproductive system Present Regionally restricted GUDMAP:9450
TS21: mesonephros of female Present Regionally restricted GUDMAP:9450
TS21: mesonephric mesenchyme of female Present Regionally restricted GUDMAP:9450 lateral expression; expression absent from the medial mesenchyme adjacent to the ovary; regional expression surrounding the nephric and paramesonephric duct epithelia both in the mesonephros and extending caudally beyond the ovary
TS21: nephric duct of female, rest of Present Regionally restricted GUDMAP:9450
TS21: mesenchyme of nephric duct of female Present GUDMAP:9450
TS21: paramesonephric duct of female, rest of Present Regionally restricted GUDMAP:9450
TS21: mesenchyme of rest of paramesonephric duct of female Present GUDMAP:9450
TS21: epithelium of rest of paramesonephric duct of female Absent GUDMAP:9450
TS21: coelomic epithelium of female mesonephros Absent GUDMAP:9450
TS21: epithelium of nephric duct of female Absent GUDMAP:9450
TS21: paramesonephric duct of female, mesonephric portion Absent GUDMAP:9450
TS21: germ cell of ovary Absent GUDMAP:9450
TS21: developing vasculature of ovary Absent GUDMAP:9450
TS21: mesonephric tubule of female Ambiguous GUDMAP:9450 possible weak expression
TS21: nephric duct of female, mesonephric portion Ambiguous GUDMAP:9450 expression is likely to be absent but the duct is obstructed by the signal in the mesenchyme
TS21: ovary Ambiguous GUDMAP:9450 uncertain weak signal on the medial surface of the ovary
Specimen GUDMAP:9451: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: urinary system Present GUDMAP:9451
TS21: metanephros Present Regionally restricted GUDMAP:9451
TS21: renal interstitium Present Regionally restricted GUDMAP:9451 signal pattern indicative of regional expression surrounding ureteric trunk; absent from the rest of the interstitium
TS21: renal pelvis Present GUDMAP:9451
TS21: ureter Present GUDMAP:9451 signal may be regional to the presumptive ureteral smooth muscle layer
TS21: ureteral mesenchyme Present GUDMAP:9451
TS21: ureteral mesenchyme middle layer Present GUDMAP:9451
TS21: early nephron Present GUDMAP:9451
TS21: renal capsule Absent GUDMAP:9451
TS21: primitive collecting duct Absent GUDMAP:9451
TS21: nephrogenic zone Ambiguous GUDMAP:9451 signal appears to be absent
Specimen GUDMAP:13632: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple. Moderate expression was seen with the low level expression control (Shh). Thus, low levels of mRNA expression should be detectable in this experiment.
Structure Level Pattern Image Note
TS23: urinary system Present GUDMAP:13632
TS23: metanephros Present GUDMAP:13632
TS23: renal cortex Present GUDMAP:13632
TS23: renal medulla Present GUDMAP:13632
TS23: renal pelvis Present GUDMAP:13632
TS23: ureter Present GUDMAP:13632
TS23: pelvic smooth muscle Present GUDMAP:13632
TS23: perihilar interstitium Present Regionally restricted GUDMAP:13632
TS23: pelvic urothelial lining Absent GUDMAP:13632
TS23: urothelium of ureter Absent GUDMAP:13632
TS23: ureteral mesenchyme Present GUDMAP:13632
TS23: ureteral vasculature Present GUDMAP:13632
TS23: ureteral smooth muscle layer Present GUDMAP:13632
TS23: ureteral smooth muscle layer smooth muscle component Present GUDMAP:13632
TS23: ureteral smooth muscle layer interstitium Present Regionally restricted GUDMAP:13632
TS23: ureteral mesenchyme outer layer Present Regionally restricted GUDMAP:13632
TS23: nephrogenic zone Present GUDMAP:13632
TS23: ureteric tip Present GUDMAP:13632
TS23: cortical collecting duct Present GUDMAP:13632
TS23: ureteric tree terminal branch excluding tip itself Present GUDMAP:13632
TS23: pretubular aggregate Absent GUDMAP:13632
TS23: cap mesenchyme Absent GUDMAP:13632
TS23: nephrogenic interstitium Absent GUDMAP:13632
TS23: comma-shaped body Ambiguous GUDMAP:13632
TS23: renal vesicle Ambiguous GUDMAP:13632
TS23: s-shaped body Present Regionally restricted GUDMAP:13632
TS23: proximal segment of s-shaped body Present GUDMAP:13632
TS23: parietal epithelium of s-shaped body Present GUDMAP:13632
TS23: distal segment of s-shaped body Ambiguous GUDMAP:13632
TS23: visceral epithelium of s-shaped body Present Regionally restricted GUDMAP:13632
TS23: medial segment of s-shaped body Absent GUDMAP:13632
TS23: renal connecting tubule of s-shaped body Absent GUDMAP:13632
TS23: capillary loop renal corpuscle Present Regionally restricted GUDMAP:13632
TS23: maturing renal corpuscle Present Regionally restricted GUDMAP:13632
TS23: cortical renal tubule Present Regionally restricted GUDMAP:13632
TS23: renal cortical interstitium Present Regionally restricted GUDMAP:13632 Only a small subset of cortical interstitium shows expression.
TS23: early tubule Present GUDMAP:13632
TS23: developing capillary loop stage nephron group Present GUDMAP:13632
TS23: maturing nephron Present GUDMAP:13632
TS23: late tubule Present GUDMAP:13632
TS23: immature loop of Henle Present GUDMAP:13632
TS23: renal medullary interstitium Present GUDMAP:13632
TS23: medullary collecting duct Absent GUDMAP:13632
TS23: renal interstitium Present GUDMAP:13632
TS23: ureteric trunk Present GUDMAP:13632
TS23: parietal epithelium of capillary loop renal corpuscle Present GUDMAP:13632
TS23: visceral epithelium of capillary loop renal corpuscle Present GUDMAP:13632
TS23: presumptive mesangium of capillary loop renal corpuscle Ambiguous GUDMAP:13632
TS23: maturing glomerular tuft Present GUDMAP:13632
TS23: glomerular mesangium of maturing glomerular tuft Ambiguous GUDMAP:13632
TS23: visceral epithelium of maturing renal corpuscle Present GUDMAP:13632
TS23: presumptive endothelium of capillary loop renal corpuscle Ambiguous GUDMAP:13632
TS23: glomerular capillary system of maturing glomerular tuft Ambiguous GUDMAP:13632
TS23: Bowman's capsule of maturing renal corpuscle Ambiguous GUDMAP:13632
TS23: renal medullary vasculature Ambiguous GUDMAP:13632
TS23: collecting duct Present GUDMAP:13632
TS23: anlage of loop of Henle of cortical renal tubule Present GUDMAP:13632
TS23: cortical early distal tubule Present GUDMAP:13632
TS23: cortical renal tubule of capillary loop nephron group Present GUDMAP:13632
TS23: cortical renal tubule of maturing nephron group Present GUDMAP:13632
TS23: cortical early proximal tubule Ambiguous GUDMAP:13632
TS23: renal connecting tubule Absent GUDMAP:13632
TS23: early distal tubule of capillary loop nephron Present GUDMAP:13632
TS23: early distal tubule of maturing nephron Present GUDMAP:13632
TS23: early proximal tubule of capillary loop nephron Ambiguous GUDMAP:13632
TS23: early proximal tubule of maturing nephron Ambiguous GUDMAP:13632
TS23: renal connecting tubule of capillary loop nephron Absent GUDMAP:13632
TS23: renal connecting tubule of maturing nephron Absent GUDMAP:13632
TS23: renal cortical vasculature Ambiguous GUDMAP:13632
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory