Antibody:	Anti-Zac1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong		3D , 3F	Expression was detected in dividing cortical progenitors. Co-expression with Pax6 was detected in most cortical progenitors.
TS20: cortical preplate	Present	Scattered	3D , 3F
TS20: cortical hem	Absent		3D , 3F	Expression was excluded from the cortical hem.
TS20: lateral ganglionic eminence	Present	Regionally restricted	3F , 3D	Expression was detected in the dorsal part of the lateral ganglionic eminence. The ventral limit of expression correlated with the ventral border for low, but not high, expression of Pax6.
TS20: medial ganglionic eminence	Absent		3F , 3D

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	3F inset right , 3D inset right	Expression was detected in a migratory stream in the basolateral telencephalon. Expression did not overlap with Pax6.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted		Co-expression with Pax6 was detected in most cortical progenitors.

Structure	Level	Pattern	Image	Note
TS20: cortical preplate	Present	Regionally restricted	3I , 3G	Expression was detected in post-mitotic neurons (TUJ1-positive).

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted		Expression was detected in post-mitotic neurons (TUJ1-positive) in deep-layers of the cortex.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted		Expression was detected in post-mitotic neurons (TUJ1-positive) in deep-layers of the cortex.