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Sox17tm4.1(icre/ERT2)Heli
Targeted Allele Detail
Summary
Symbol: Sox17tm4.1(icre/ERT2)Heli
Name: SRY (sex determining region Y)-box 17; targeted mutation 4.1, Heiko Lickert
MGI ID: MGI:5543602
Synonyms: Sox17CreERT2, Sox17CreERT2deltaneo
Gene: Sox17  Location: Chr1:4561154-4567577 bp, - strand  Genetic Position: Chr1, 1.65 cM
Alliance: Sox17tm4.1(icre/ERT2)Heli page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:204611
Parent Cell Line:  IDG3.2 (ES Cell)
Strain of Origin:  (C57BL/6J x 129S6/SvEvTac)F1
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Sox17tm4.1(icre/ERT2)Heli expression driven by 1 gene
 
Mutation details: A targeting vector was designed to delete the translational stop codon of Sox17 and to introduce the codon-optimized cre fused to the triple-mutant (G400V, M543A and L544A) human estrogen receptor ligand binding domain (ERT2) sequence preceded by a viral 2A peptide sequence into exon 5. Homologous recombination in ES cells integrated the cre/ERT2 cassette and Frt-flanked neomycin marker so that the targeted Sox17 allele will produce an bicistronic mRNA that will be co-translationally cleaved into equimolar amounts of Sox17 and cre. Chimeras were crossed to a Flp deleter line to remove the neo selection marker. (J:204611)
Generation of Sox17tm4.1(icre/ERT2)Heli.
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Sox17 (mouse)
Summary of all recombinase alleles driven by Sox17.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sox17 Mutation:  29 strains or lines available
References
Original:  J:204611 Engert S, et al., The Sox17CreERT2 knock-in mouse line displays spatiotemporal activation of Cre recombinase in distinct Sox17 lineage progenitors. Genesis. 2013 Nov;51(11):793-802
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory