Antibody:	Anti-Nestin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS18: brain	Present	Regionally restricted	1A	Expression was detected in radial glial cells.

Structure	Level	Pattern	Image	Note
TS18: pancreas primordium	Weak	Clusters	1B , 1C	Expression was low in clusters of cells. Most cells in the clusters co-expressed Glucagon and Nestin.

Structure	Level	Pattern	Image	Note
TS18: pancreas primordium	Present			Cells co-expressing Nestin and Glucagon were observed in the mutant.

Structure	Level	Pattern	Image	Note
TS18: pancreas primordium	Present			Cells co-expressing Nestin and Glucagon were observed in the mutant.

Structure	Level	Pattern	Image	Note
TS18: pancreas primordium	Present		1F , 1E	An occasional Insulin-positive cell co-expressed Nestin.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	2A	Co-expression with insulin was noted in a large number of cells. 30% of insulin-positive cells expressed nestin. Expression was detected in many exocrine cells.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Present	Regionally restricted	2B	Co-expression with Glucagon was noted in a significant number of cells.

Structure	Level	Pattern	Image	Note
TS23: pancreas	Absent		2D

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		3A	The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the PC2 P1 islets.

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present			The number of cells co-expressing Nestin and Insulin decreased abruptly and were not observed in the Gcgr mutant P1 islets.

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present		3B	The number of cells co-expressing Nestin and Glucagon decreased abruptly and were not observed in the PC2 mutant P1 islets.

Structure	Level	Pattern	Image	Note
TS27: pancreas	Present			The number of cells co-expressing Nestin and Glucagon decreased abruptly and were not observed in the Gcgr mutant P1 islets.

Structure	Level	Pattern	Image	Note
TS28: exocrine pancreas	Present	Single cells

Structure	Level	Pattern	Image	Note
TS27: exocrine pancreas	Present		3D , 3E	A large number of immunopositive cells were observed in the exocrine compartment of the PC2 mutant. Co-expression with Amylase was noted.

Structure	Level	Pattern	Image	Note
TS27: exocrine pancreas	Present			A large number of immunopositive cells were observed in the exocrine compartment of the Gcgr mutant. Co-expression with Amylase was noted.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4A	Expression was detected in large numbers of cells in aproximately 10% of the islets and some of these cells co-expressed Insulin.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4C	Expression was detected in large numbers of cells in aproximately 10% of the islets and some of these cells co-expressed Glucagon.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4D	Expression was detected in large numbers of cells in aproximately 10% of the islets and some of these cells co-expressed Somatostatin.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4E	Expression was detected in large numbers of cells in aproximately 10% of the islets and some of these cells co-expressed Pancreatic Polypeptide.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4F	All islets contained immunopositive cells that did not co-express hormones.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Present	Regionally restricted	4G	All islets contained immunopositive cells that did not co-express hormones.

Structure	Level	Pattern	Image	Note
TS28: exocrine pancreas	Present		5A	A large number of immunopositive cells were detected in the Gcgr-/- pancreas.
TS28: islets of Langerhans	Present	Regionally restricted	5A	Expression was localized to a subset of pancreatic lobules.
TS28: pancreas	Present	Clusters	5A	Expression was clustered in some lobules.

Structure	Level	Pattern	Image	Note
TS28: exocrine pancreas	Present			Expression was detected in a large number of cells in the exocrine compartment of PC2-/- pancreas.

Structure	Level	Pattern	Image	Note
TS28: endocrine pancreas	Trace		5B	There was a near absence of immunopositive cells in the wild type.
TS28: exocrine pancreas	Trace		5B	There was a near absence of immunopositive cells in the wild type.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present	Scattered	5C

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present		5D	A large number of immunopositive cells were detected in the Gcgr-/- pancreas. Expression was clustered in some lobules.

Structure	Level	Pattern	Image	Note
TS28: pancreas	Present		5E	Many immunopositive cells co-express Amylase.