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Gene Expression Data
Assay Details
Assay
Reference: J:169913 Hudson LD, et al., A tool for examining the role of the zinc finger myelin transcription factor 1 (Myt1) in neural development: Myt1 knock-in mice. Transgenic Res. 2011 Aug;20(4):951-61
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5553340
Gene symbol: Myt1
Gene name: myelin transcription factor 1
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: The fixative included picric acid.
Results
Specimen 4A/C +/-: embryonic day 18.0; Myt1tm1(EGFP/icre)Ldh/Myt1+ (more )
Note: Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
Structure Level Pattern Image Note
TS26: spinal cord Present Widespread 4C +/- , 4A +/- Labeled cells are observed throughout the spinal cord. Similar numbers of labeled cells are seen in heterozygotic and homozygotic animals. Cells do not double-label with a neural marker, indicating these are undifferentiated neural progenitors.
Specimen 4A/C -/-: embryonic day 18.0; Myt1tm1(EGFP/icre)Ldh/Myt1tm1(EGFP/icre)Ldh (more )
Note: Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
Structure Level Pattern Image Note
TS26: spinal cord Present Widespread 4A -/- , 4C -/- Labeled cells are observed throughout the spinal cord. Similar numbers of labeled cells are seen in heterozygotic and homozygotic animals. Cells do not double-label with a neural marker, indicating these are undifferentiated neural progenitors.
Specimen 5: embryonic day 18.0; Myt1tm1(EGFP/icre)Ldh/0 (more )
Note: Double labeled: green - Myt1 reporter; red - Rbfox3 (assay MGI:5553348).
Structure Level Pattern Image Note
TS26: spinal cord Present 5 Labeled cells are observed throughout the spinal cord. These cells are not double-labeled with a marker for mature neurons.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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