Tg(hs752-Ncre,-lacZ)1Fawa
Transgene Detail
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Symbol: |
Tg(hs752-Ncre,-lacZ)1Fawa |
Name: |
transgene insertion 1, Fan Wang |
MGI ID: |
MGI:5556205 |
Synonyms: |
CreN752 |
Transgene: |
Tg(hs752-Ncre,-lacZ)1Fawa Location: unknown
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Alliance: |
Tg(hs752-Ncre,-lacZ)1Fawa page
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Strain of Origin: |
Not Specified
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Transgene Type: |
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Transgenic (Recombinase) |
Mutation: |
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Insertion
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Tg(hs752-Ncre,-lacZ)1Fawa expression driven by
1 gene
Transgene expression driven by:
Organism |
Driver Gene |
Note |
human |
hs752 |
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Mutation details: The N-terminal fragment of cre recombinase (aa 19-59) fused to the intein-N peptide fragment (123 N terminal amino acids of the DnaE split intein sequence was cloned into a vector containing the human enhancer 752 sequence (hs752) and an Hsp68 minimal promoter and lacZ reporter. The final construct included an IRES sequence separating the lacZ gene and Ncre-intein-N. Injection of the linearized construct into pronuclei was performed to generated transgenic founders. Line numbers were not identified in the publication.
(J:207270)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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hs752
(human)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Examples of reporter expression driven by this and other human enhancers in mouse embryos produced by transient transgenesis can be viewed at http://enhancer.lbl.gov.
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Original: |
J:207270 Wang P, et al., Intersectional Cre driver lines generated using split-intein mediated split-Cre reconstitution. Sci Rep. 2012;2:497 |
All: |
1 reference(s) |
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