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Tg(Ins1-cre)25Utr
Transgene Detail
Summary
Symbol: Tg(Ins1-cre)25Utr
Name: transgene insertion 25, Ken-ichi Yagami
MGI ID: MGI:5560759
Synonyms: BAC Ins1-cre25
Transgene: Tg(Ins1-cre)25Utr  Location: unknown  Genetic Position: Chr15, Syntenic
Alliance: Tg(Ins1-cre)25Utr page
Transgene
origin
Strain of Origin:  C57BL/6N
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Tg(Ins1-cre)25Utr expression driven by 1 gene
 
Mutation details: The targeting vector contained homology arms flanking a nuclear localization signal (NLS) fused to a fragment encoding cre recombinase with a polyadenylation signal. Red/ET recombination in bacterial cells inserted the NLS-cre sequence at the translation initiation site in exon 2 of the Ins1 gene located in a BAC (RP23-181I21). The engineered BAC was linearized and injected into pronuclei of fertilized C57BL/6N oocytes. Five founders had the inserted transgnene (#'s 7, 24, 25, 28 and 32). Line 25 was used further as recombinase reporter activity in the pancreas in F1 crosses was greater than that from other founders. (J:205139, J:208032)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Ins1 (mouse)
Summary of all recombinase alleles driven by Ins1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:208032 Hasegawa Y, et al., Generation and Characterization of Ins1-cre-driver C57BL/6N for Exclusive Pancreatic Beta Cell-specific Cre-loxP Recombination. Exp Anim. 2014;63(2):183-91
All:  7 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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11/12/2024
MGI 6.24 		The Jackson Laboratory