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Trhtm1.1(cre)Mjkr
Targeted Allele Detail
Summary
Symbol: Trhtm1.1(cre)Mjkr
Name: thyrotropin releasing hormone; targeted mutation 1.1, Michael J Krashes
MGI ID: MGI:5562874
Synonyms: Trh-IRES-Cre
Gene: Trh  Location: Chr6:92219042-92221631 bp, - strand  Genetic Position: Chr6, 41.03 cM
Alliance: Trhtm1.1(cre)Mjkr page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:207472
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Trhtm1.1(cre)Mjkr expression driven by 1 gene
 
Mutation details: Using recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to cre and an frt-flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 2 of the Trh gene in a BAC. This construct was used to isolate a targeting plasmid with the cre-kanamycin cassette with upstream and downstream Trh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals, however, a partial neo (164 bp fragment of the 3' end of neo) remains between the IRES-Cre and polyA sequences. (J:101977, J:207472)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Trh (mouse)
Summary of all recombinase alleles driven by Trh.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Trh Mutation:  23 strains or lines available
References
Original:  J:207472 Krashes MJ, et al., An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger. Nature. 2014 Mar 13;507(7491):238-42
All:  8 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
10/09/2024
MGI 6.24 		The Jackson Laboratory