Tg(Acan-cre/ERT2,-luc)1Gbg
Transgene Detail
|
|
| Symbol: |
Tg(Acan-cre/ERT2,-luc)1Gbg |
| Name: |
transgene insertion 1, George Bou-Gharios |
| MGI ID: |
MGI:5563229 |
| Synonyms: |
Acan-CreER-ires-Luc |
| Transgene: |
Tg(Acan-cre/ERT2,-luc)1Gbg Location: unknown
|
| Alliance: |
Tg(Acan-cre/ERT2,-luc)1Gbg page
|
|
| Strain of Origin: |
Not Specified
|
|
| Transgene Type: |
|
Transgenic (Inducible, Recombinase, Reporter) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Tg(Acan-cre/ERT2,-luc)1Gbg expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Acan |
|
|
| |
|
Mutation details: A construct was generated where four tandem repeats of the A1 enhancer of the aggrecan gene (-9,477 bp to -9,118 bp of Acan) were cloned upstream of the Col2a1 proximal promoter fragment. The cre/ERT2 sequence, followed by an IRES site and the firefly luciferase cDNA, were inserted after these regulatory elements. The construct was validated by transfection into MEFs isolated from Rosa-lacZ reporter mice, and then used to produce transgenic mice. Founders were crossed with reporter-expressing mice and three showed cre-mediated reporter activity. One line was used for further experiments.
(J:208367)
|
|
Generation of Tg(Acan-cre/ERT2,-luc)1Gbg. |
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Acan
(species not specified)
|
| |
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:208367 Lo Cascio L, et al., Generation of a mouse line harboring a Bi-transgene expressing luciferase and tamoxifen-activatable creER(T2) recombinase in cartilage. Genesis. 2014 Feb;52(2):110-9 |
| All: |
2 reference(s) |
|
Analysis Tools
