Gene Expression Data
Assay Details
Assay
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Reference:
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J:137171
Shay EL, et al., Dynamic expression patterns of ECM molecules in the developing mouse olfactory pathway. Dev Dyn. 2008 Jul;237(7):1837-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5563371
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Gene symbol:
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Hspg2
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Gene name:
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perlecan (heparan sulfate proteoglycan 2)
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Results
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Specimen
2B E13:
embryonic day 13.5
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B E13:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Specimen
2B E15:
embryonic day 15.5
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B E15:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: blood vessel |
Present |
Regionally restricted |
2B E15
|
Expression was detected in the basement membranes of blood vessels.
|
Specimen
2B E17:
embryonic day 17.5
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B E17:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 17.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: blood vessel |
Present |
Regionally restricted |
2B E17
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Expression was detected in the basement membranes of blood vessels.
|
TS25: main olfactory bulb |
Present |
Regionally restricted |
2B E17
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Expression was detected in the olfactory nerve layer in the olfactory bulb.
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Specimen
2B PND0:
postnatal day 0
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B PND0:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 0
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: blood vessel |
Present |
Regionally restricted |
2B PND0
|
Expression was detected in the basement membranes of blood vessels.
|
Specimen
2B PND2:
postnatal day 2
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B PND2:
(close )
Genetic Background:
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CD-1
|
Age:
|
postnatal day 2
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: blood vessel |
Present |
Regionally restricted |
2B PND2
|
Expression was detected in the basement membranes of blood vessels.
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Specimen
2B PND4:
postnatal day 4
(more )
Note: Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
Specimen
2B PND4:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Hspg2; blue - Ncam1 (assay MGI:5563046).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: blood vessel |
Present |
Regionally restricted |
2B PND4
|
Expression was detected in the basement membranes of blood vessels.
|
Specimen
3C:
embryonic day 15.5
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: olfactory epithelium |
Present |
Regionally restricted |
3C
|
Expression was detected in the basement membrane of the olfactory epithelium.
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TS23: nasal septum lamina propria |
Present |
Regionally restricted |
3C
|
Expression was detected in the lamina propria surrounding the fascicles of olfactory nerve axons labeled with Ncam.
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Specimen
3D:
postnatal day 2
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
3D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory epithelium |
Present |
Regionally restricted |
3D
|
Expression was detected in the basement membrane of the olfactory epithelium.
|
TS27: nasal septum lamina propria |
Present |
Regionally restricted |
3D
|
Expression was detected in the lamina propria surrounding the fascicles of olfactory nerve axons labeled with Ncam.
|
Specimen
4C:
embryonic day 15.5
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
4C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: olfactory nerve |
Present |
Regionally restricted |
4C
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Expression was detected in the olfactory ensheathing cells surrounding the olfactory sensory neuron axons crossing the cribriform plate. Expression was not seen surrounding every bundle at E15.5.
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Specimen
4D:
postnatal day 2
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
4D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory nerve |
Present |
Regionally restricted |
4D
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Expression was detected in the olfactory ensheathing cells surrounding the olfactory sensory neuron axons crossing the cribriform plate. At P2, expression appeared to surround all axon bundles.
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Specimen
5C:
embryonic day 15.5
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
5C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: olfactory nerve |
Present |
Regionally restricted |
5C
|
At E15.5, expression was found surrounding the entry zone, where the olfactory nerve enters the olfactory bulb. However, there was no expression in olfactory ensheathing cells within the entry zone.
|
TS23: blood vessel |
Present |
Regionally restricted |
5C
|
Expression was detected in the basement membrane of blood vessels in the entry zone of the olfactory nerve fascicles as they enter the olfactory bulb.
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Specimen
5D:
postnatal day 2
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
5D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: olfactory nerve |
Present |
Regionally restricted |
5D
|
At P2, expression was detected surrounding olfactory nerve fascicles within the entry zone, where the olfactory nerve enters the olfactory bulb.
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Specimen
6C:
embryonic day 15.5
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
6C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: main olfactory bulb |
Absent |
|
6C
|
Expression was not detected in the olfactory nerve layer in the olfactory bulb.
|
Specimen
6D:
postnatal day 2
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
Specimen
6D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - Tnc (assay MGI:5563418).
|
Specimen
7A:
embryonic day 15.5
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - laminin.
Specimen
7A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - laminin.
|
Specimen
7B:
postnatal day 2
(more )
Note: Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - laminin.
Specimen
7B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Hspg2; blue - Ncam1 (assay MGI:5563046); green - laminin.
|
|