Probe:	IMAGE clone 445088
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	1A	Expression was detected in the anterior neural plate.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	1B	Expression was detected in the anterior forebrain.
TS14: future diencephalon	Present	Regionally restricted	1B	Expression was detected in the ventral diencephalon.

Structure	Level	Pattern	Image	Note
TS15: lung	Present		1C
TS15: olfactory placode	Present		1C
TS15: optic stalk	Present		1C
TS15: optic vesicle	Present		1C

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	1D	Expression was detected in the ventral forebrain.
TS17: Rathke's pouch	Present		1D
TS17: optic stalk	Present		1D
TS17: infundibular recess of 3rd ventricle	Present	Regionally restricted	1D	Expression was detected in the infundibulum.
TS17: nasal pit	Present	Regionally restricted	1D	Expression was detected in the olfactory placode.

Structure	Level	Pattern	Image	Note
TS15: optic cup inner layer	Present		2A	Expression was detected in the presumptive neural retina.
TS15: optic stalk	Present		2A
TS15: optic vesicle	Present		2A
TS15: optic cup outer layer	Absent		2A	Expression was not in the future retinal pigment epithelium.
TS15: lens placode	Absent		2A	Expression was not in the lens ectoderm.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present		2B
TS19: optic stalk	Present		2B

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	2C	Expression was decreased in the presumptive ganglion cell layer, but was maintained in the progenitor zone.
TS21: optic stalk	Present	Regionally restricted	2C	Expression was detected in the dorsal optic stalk.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted		Expression was detected in the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	2D	Expression was restricted to the neuroblastic layer.
TS27: retina outer nuclear layer	Weak	Non-Uniform	2D	Expression was downregulated, and the expression pattern was mottled.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	3A	Expression was detected in the anterior neural plate.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	3B	Expression was detected in the ventral forebrain.
TS14: stomatodeum ectoderm	Present	Regionally restricted	3B	Expression was detected in the oral ectoderm.

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Present	Regionally restricted	3C	Expression was detected along the midline of the ventral forebrain.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	3D	Expression was detected in the ventral forebrain.
TS17: oral region	Present	Regionally restricted	3D	Expression was detected in oral ectoderm.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present	Regionally restricted	3E	Expression was detected along the midline of the ventral forebrain.
TS17: Rathke's pouch	Present		3E

Structure	Level	Pattern	Image	Note
TS17: nasal pit	Present	Regionally restricted	3F	Expression was detected in the medial olfactory placode.

Structure	Level	Pattern	Image	Note
TS19: infundibular recess of 3rd ventricle	Present			Expression was detected in the developing infundibulum.
TS19: Rathke's pouch	Ambiguous			Expression was down-regulated in Rathke's pouch.
TS19: forebrain	Ambiguous	Regionally restricted		Expression was down-regulated in the ventral forebrain.
TS19: olfactory epithelium	Ambiguous	Regionally restricted		Expression was down-regulated in the medial olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Strong	Regionally restricted	S3B	Strong expression was detected in the distal optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	S3C	Expression was reduced in the distal optic vesicle of the conditional mutant compared to the control.