Spp2<tm1(cre/ERT2)Amc> Targeted Allele Detail MGI Mouse (MGI:5565344)

Spp2^{tm1(cre/ERT2)Amc}
Targeted Allele Detail

Summary

Symbol:	Spp2^{tm1(cre/ERT2)Amc}
Name:	secreted phosphoprotein 2; targeted mutation 1, Andrew P McMahon
MGI ID:	MGI:5565344
Synonyms:	Spp2^G2aCE, Spp2^{tm1(GFP,cre/ERT2)Amc}
Gene:	Spp2 Location: Chr1:88334683-88354160 bp, + strand Genetic Position: Chr1, 44.63 cM, cytoband C5
Alliance:	Spp2^{tm1(cre/ERT2)Amc} page

Mutation
origin

Mutant Cell Line:	EPD0558_1_A04
Germline Transmission:	Earliest citation of germline transmission: J:159099
Parent Cell Line:	JM8A3.N1 (ES Cell)
Strain of Origin:	C57BL/6N-A^tm1Brd

Mutation
description

Allele Type:

Targeted (Inducible, Null/knockout, RMCE-ready, Recombinase, Reporter)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion Vector: L1L2_Bact_P

Spp2^{tm1(cre/ERT2)Amc} expression driven by 1 gene

Mutation details: The allele was generated by a dual recombinase-mediated cassette exchange protocol. The exchange vector contained (from 5' to 3') an frt site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein (EGFP) sequence, a viral T2A oligopeptide, and a cre/ERT2 sequence. A rox-flanked puromycin resistance (puro) cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Spp2 (mouse) Summary of all recombinase alleles driven by Spp2. Alliance mouse Spp2 gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Spp2 Mutation:	20 strains or lines available

Notes

This exchange vector (along with the pDIRE plasmid containing an icre-expression cassette and a Flpo-expression cassette to facilitate insertion of the exchange vector) was electroporated into C57BL/6N-Atm1Brd-derived JM8A3.N1 knockout first reporter embryonic stem (ES) cells obtained from the European Conditional Mouse Mutagenesis (EUCOMM) consortium containing clone EPDO558_1_A04). Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony. Recombined ES cells, with the neo removed and resistant to puro, were injected into B6(Cg)-Tyrc-2J/J blastocysts and resulting chimeric mice were bred to C57BL/6J to establish a colony.

References

Original:	J:159099 McMahon A, et al., Summary of mouse strains characterized by GUDMAP consortium. Unpublished. 2010;
All:	1 reference(s)

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