Hprt1<tm357(Ple278-icre/ERT2)Ems> Targeted Allele Detail MGI Mouse (MGI:5568229)

Hprt1^{tm357(Ple278-icre/ERT2)Ems}
Targeted Allele Detail

Summary

Symbol:	Hprt1^{tm357(Ple278-icre/ERT2)Ems}
Name:	hypoxanthine phosphoribosyltransferase 1; targeted mutation 357, Elizabeth M Simpson
MGI ID:	MGI:5568229
Synonyms:	KCNA4-creERT2, mEMS6120, Ple278-icre/ERT2
Gene:	Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance:	Hprt1^{tm357(Ple278-icre/ERT2)Ems} page

Mutation
origin

Mutant Cell Line:	mEMS6120
Germline Transmission:	Earliest citation of germline transmission: J:204202
Parent Cell Line:	mEMS4855.02 (ES Cell)
Strain of Origin:	B6N.Cg-A^w-J Hprt1^b-m3
Project Collection:	EUCOMMTOOLS

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Hprt1^{tm357(Ple278-icre/ERT2)Ems} expression driven by 1 gene

Mutation details: The transgene consists of a RP11-63.H13 human bacterial artificial chromosome (BAC) retrofitted to contain a human HPRT complementary sequence (containing exon 1, intron 1, exon 2 and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm; all inserted into the sacB gene in the BAC vector. Next, an iCre/ERT2 fusion gene and an frt-flanked kanamycin cassette were inserted directly at the ATG of the human KCNA4 locus. These transgene is targeted as a single copy "knock-in" into the upstream region of the Hprt locus. Transient infection with FLP recombinase removes the kanamycin cassette. (J:270567)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	KCNA4 (human) Summary of all recombinase alleles driven by KCNA4. Alliance human KCNA4 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:	1279 strains or lines available

References

Original:	J:204202 The Pleiades Promoter and CanEuCre Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2014;
All:	2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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