Th<tm1.1(cre/ERT2)Ddg> Targeted Allele Detail MGI Mouse (MGI:5569743)

Th^{tm1.1(cre/ERT2)Ddg}
Targeted Allele Detail

Summary

Symbol:	Th^{tm1.1(cre/ERT2)Ddg}
Name:	tyrosine hydroxylase; targeted mutation 1.1, David Ginty
MGI ID:	MGI:5569743
Synonyms:	TH^2A-CreER
Gene:	Th Location: Chr7:142446516-142453732 bp, - strand Genetic Position: Chr7, 88.06 cM
Alliance:	Th^{tm1.1(cre/ERT2)Ddg} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:239228
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S6/SvEvTac

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutation:

Insertion

Th^{tm1.1(cre/ERT2)Ddg} expression driven by 1 gene

Mutation details: A T2A cleavage peptide and the cre/ERT2 fusion protein-coding sequence were introduced to the 3'UTR of the mouse Th gene. An FRT-PGK-EM7-NEOpolyA-FRT-loxP cassette was placed downstream of the 3'UTR region. Homologous recombination was achieved in 129S6/SvEvTac-derived embryonic stem cells and resultant chimeric mice were crossed with Flp mice to excise the neomycin cassette. (J:239228)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Th (mouse) Summary of all recombinase alleles driven by Th. Comparative matrix view of recombinase activities Alliance mouse Th gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Th Mutation:	58 strains or lines available

References

Original:	J:239228 Abraira VE, et al., The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell. 2017 Jan 12;168(1-2):295-310.e19
All:	19 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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