Tg(Tg-cre/ERT2)1Kero
Transgene Detail
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| Symbol: |
Tg(Tg-cre/ERT2)1Kero |
| Name: |
transgene insertion 1, Jukka Kero |
| MGI ID: |
MGI:5575412 |
| Synonyms: |
TgCreERT2 |
| Transgene: |
Tg(Tg-cre/ERT2)1Kero Location: unknown
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| Alliance: |
Tg(Tg-cre/ERT2)1Kero page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Tg-cre/ERT2)1Kero expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Tg |
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Mutation details: RedE/RedT-recombination was used to insert a cassette encoding tamoxifen-inducible cre (cre/ERT2), an frt-flanked ampicillin selection marker, and the bovine growth hormone polyadenylation signal at the ATG start codon of the thyroglobulin (Tg) gene in a P1 artificial chromosome (PAC). Following recombination, flp-mediated deletion of the ampicillin marker was performed. The verified construct was linearized, then used for injection of C57BL/6 fertilized oocytes. 5/35 pups tested positive for cre by PCR. Founders were tested for specificity and inducibility of cre recombinase by breeding to lacZ reporter mice and treating double mutants with tamoxifen. 4 founders showed cre activity only in the thyroid gland, and one line with strong and homogenous cre activity was chosen for detailed analyses.
(J:211398)
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Generation of Tg(Tg-cre/ERT2)1Kero. |
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tg
(species not specified)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:211398 Undeutsch H, et al., A mouse model with tamoxifen-inducible thyrocyte-specific cre recombinase activity. Genesis. 2014 Apr;52(4):333-40 |
| All: |
6 reference(s) |
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Analysis Tools
