Insm1<tm1.1(GFP/cre)Mgn> Targeted Allele Detail MGI Mouse (MGI:5576020)

Insm1^{tm1.1(GFP/cre)Mgn}
Targeted Allele Detail

Summary

Symbol:	Insm1^{tm1.1(GFP/cre)Mgn}
Name:	insulinoma-associated 1; targeted mutation 1.1, Mark A Magnuson
MGI ID:	MGI:5576020
Synonyms:	Insm1(GFP-Cre), Insm1^GFPCre, Insm1^tm1.1Mgn
Gene:	Insm1 Location: Chr2:146064021-146066940 bp, + strand Genetic Position: Chr2, 71.33 cM
Alliance:	Insm1^{tm1.1(GFP/cre)Mgn} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:94077
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S6/SvEvTac

Mutation
description

Allele Type:

Targeted (Recombinase, Reporter)

Mutation:

Insertion

Insm1^{tm1.1(GFP/cre)Mgn} expression driven by 1 gene

Mutation details: Using recombinase mediated cassette exchange (RMCE) a 532 bp fragment containing a GFP-Cre fusion protein replaced the entire coding region of the gene. The donor construct includes a frt-flanked pgk-hygromycin immediately downstream of the GFP-Cre fusion sequence. The frt-flanked pgk-hygromycin was removed by FLPe mediated recombination. Western blot analysis confirmed absence of protein in homozygotes. Green fluorescence is seen in pancreatic pre-endocrine cells and in islet cells after birth, in the developing central nervous system between E9.5 and E18.5, and also in the peripheral nervous system and gut endocrine cells. (J:94077, J:214095)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Insm1 (mouse) Summary of all recombinase alleles driven by Insm1. Alliance mouse Insm1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	15 assay results 3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Insm1 Mutation:	13 strains or lines available

References

Original:	J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015;
All:	8 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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