Tg(DMD)72Thoen
Transgene Detail
|
|
| Symbol: |
Tg(DMD)72Thoen |
| Name: |
transgene insertion 72, Peter 't Hoen |
| MGI ID: |
MGI:5578554 |
| Synonyms: |
hDMD |
| Transgene: |
Tg(DMD)72Thoen Location: unknown Genetic Position: Chr5, Syntenic
|
| Alliance: |
Tg(DMD)72Thoen page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:132320
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
(DBA/2 x 129P2/OlaHsd)F1
|
|
| Transgene Type: |
|
Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
|
Insertion
|
| |
|
Tg(DMD)72Thoen expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Homolog in Mouse |
Note |
| human |
DMD (1756) |
|
|
|
| |
|
Mutation details: The construct consists of a 2.7 Mbp yeast artificial chromosome (YAC) sequence containing the full-length 2.3 Mbp human dystrophin gene (DMD) and a hygromycin resistance cassette on one end and a neomycin resistance cassette on the other. Spheroplast fusion of YAC-containing yeast cells with murine (DBA2 x 129OLA)F1-derived embryonic stem (ES) cells resulted in ES cells with the integration of the transgene as a tail to tail duplication into mouse chromosome 5. Analysis by Western blot and human-specific antibody demonstrated the presence of full length protein at the sarcolemma.
(J:132320)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 4 strains available
Cell Lines: 0 lines available
|
|
| Original: |
J:132320 't Hoen PA, et al., Generation and characterization of transgenic mice with the full-length human DMD gene. J Biol Chem. 2008 Feb 29;283(9):5899-907 |
| All: |
6 reference(s) |
|
Analysis Tools
