Col1a1<tm3(tetO-Fosl1,-DsRed)Wag> Targeted Allele Detail MGI Mouse (MGI:5585716)

Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag}
Name:	collagen, type I, alpha 1; targeted mutation 3, Erwin F Wagner
MGI ID:	MGI:5585716
Synonyms:	tet-O-Fosl1, tet-O-Fra1
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:213764
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence, RMCE-ready, Reporter)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm3(tetO-Fosl1,-DsRed)Wag} expresses 1 gene

Mutation details: KH2 ES cells were retargeted with a "flp-in plasmid containing a tetracycline responsive element (TRE or tetO), FLAG-tagged Fosl1 cDNA sequence, GtX IRES sequence and DsRed reporter. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. (J:213764)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:213764 Hasenfuss SC, et al., Activator Protein 1 transcription factor Fos-related antigen 1 (Fra-1) is dispensable for murine liver fibrosis, but modulates xenobiotic metabolism. Hepatology. 2014 Jan;59(1):261-73
All:	3 reference(s)

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