Col1a1<tm4(tetO-Jun/Fosl2)Wag> Targeted Allele Detail MGI Mouse (MGI:5586533)

Col1a1^{tm4(tetO-Jun/Fosl2)Wag}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm4(tetO-Jun/Fosl2)Wag}
Name:	collagen, type I, alpha 1; targeted mutation 4, Erwin F Wagner
MGI ID:	MGI:5586533
Synonyms:	Col1a1^{TetOP-c-Jun~Fosl2}
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm4(tetO-Jun/Fosl2)Wag} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:210545
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence, RMCE-ready)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm4(tetO-Jun/Fosl2)Wag} expresses 2 genes

Mutation details: KH2 ES cells were retargeted with a "flp-in plasmid" containing a tetracycline responsive element (TRE or tetO) and a forced dimerization fusion cDNA of Jun lacking the stop codon and Fosl2 connected by a flexible short polyGly-Ser peptide with a C-terminal FLAG-tag. A flpe-recombinase plasmid facilitated integration into the 3' UTR region. (J:210545)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:210545 Hasenfuss SC, et al., Regulation of steatohepatitis and PPARgamma signaling by distinct AP-1 dimers. Cell Metab. 2014 Jan 7;19(1):84-95
All:	1 reference(s)

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