Probe:	plexin-A4 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS16: dorsal root ganglion	Present
TS16: trigeminal ganglion	Present
TS16: peripheral nervous system	Present	Regionally restricted		Expression was detected in the sympathetic ganglion.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		3A
TS20: basal columns	Present	Regionally restricted	3A	Expression was detected in a subset of motor neurons in the spinal cord.
TS20: alar columns	Present	Regionally restricted	3A	Expression was detected in the dorsal horn.

Structure	Level	Pattern	Image	Note
TS24: trigeminal ganglion	Strong		3B

Structure	Level	Pattern	Image	Note
TS24: inferior glossopharyngeal ganglion	Strong		3C
TS24: inferior vagus ganglion	Strong		3C
TS24: superior glossopharyngeal ganglion	Strong		3C
TS24: superior vagus ganglion	Strong		3C

Structure	Level	Pattern	Image	Note
TS24: sympathetic ganglion	Strong		3D
TS24: brainstem	Present	Regionally restricted	3D	Expression was detected in the facial nucleus and the sensory trigeminal nuclei.

Structure	Level	Pattern	Image	Note
TS24: sympathetic ganglion	Strong	Regionally restricted	3E	Expression was strong in the celiac ganglion.

Structure	Level	Pattern	Image	Note
TS24: enteric nervous system	Strong	Regionally restricted	3F	Expression was strong in enteric neurons in the digestive tract.

Structure	Level	Pattern	Image	Note
TS24: ciliary ganglion	Strong
TS24: facial ganglion	Strong
TS24: otic ganglion	Strong
TS24: sphenopalatine ganglion	Strong
TS24: submandibular ganglion	Strong
TS24: amygdala	Present	Regionally restricted		Expression was detected in the anterior cortical and medial amygdaloid nuclei.

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	3G	Expression was detected in the motor trigeminal nucleus and the oculomotor nucleus.
TS24: superior colliculus	Weak		3G

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	3H	Expression was detected in the nucleus ambiguus, the dorsal nucleus of the vagus, and the hypoglossal nucleus.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	3I	Expression was detected in the ventral posterolateral and posteromedial thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present		4A
TS24: main olfactory bulb	Absent		4A
TS24: vomeronasal organ epithelium	Absent		4A

Structure	Level	Pattern	Image	Note
TS24: olfactory cortex	Present	Non-Uniform	4B	Expression was strong in the anterior olfactory nucleus and also detected in the bed nucleus of the accessory olfactory tract.
TS24: main olfactory bulb	Absent		4B

Structure	Level	Pattern	Image	Note
TS24: cochlear ganglion	Strong		4E
TS24: vestibular ganglion	Strong		4E
TS24: organ of Corti	Present		4E
TS24: saccule	Present	Regionally restricted	4E	Expression was detected in the maculae.
TS24: utricle	Present	Regionally restricted	4E	Expression was detected in the maculae.

Structure	Level	Pattern	Image	Note
TS24: brainstem	Present	Regionally restricted	4F	Expression was detected in the facial nucleus, the sensory trigeminal nuclei, and the medial, superior and spinal vestibular nuclei. Moderate expression was noted in the cochlear nucleus.
TS24: labyrinth epithelium	Present		4F
TS24: vestibular component epithelium	Absent		4F	Expression was not detected in the ampullary crests.
TS24: inferior colliculus	Moderate		4F

Structure	Level	Pattern	Image	Note
TS23: cortical plate	Strong	Regionally restricted	5A	Strong expression was detected in the superficial part of the cortical plate. Expression was weaker in the deeper part of the cortical plate.
TS23: cerebral cortex intermediate zone	Weak		5A
TS23: cerebral cortex subventricular zone	Weak		5A
TS23: cerebral cortex ventricular layer	Absent		5A

Structure	Level	Pattern	Image	Note
TS27: neocortex layer	Strong	Regionally restricted	5B	Strong expression was detected in the superficial part of the cortical plate. Expression was weaker in the deeper part of the cortical plate.
TS27: cerebral cortex intermediate zone	Weak		5B
TS27: cerebral cortex subventricular zone	Weak		5B
TS27: cerebral cortex ventricular zone	Absent		5B

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Moderate		5C

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present	Regionally restricted	5D	Expression was strong in the stratum pyramidalis of the cornu ammonis (CA) 1 field but weak in the CA3 field and the dentate gyrus.

Structure	Level	Pattern	Image	Note
TS27: dentate gyrus	Present		5E
TS27: hippocampus CA1	Present		5E
TS27: hippocampus CA3	Present		5E

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		5F
TS28: hippocampus region	Weak		5F	Expression was slightly down-regulated in the CA regions.