Antibody:	Anti-Ncoa4 (H-300) sc-28749
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: primitive ventricle cardiac muscle	Present		4A E9.5

Structure	Level	Pattern	Image	Note
TS17: primitive ventricle cardiac muscle	Present		4A E10.5

Structure	Level	Pattern	Image	Note
TS19: ventricle cardiac muscle	Strong		4A E11.5

Structure	Level	Pattern	Image	Note
TS20: ventricle cardiac muscle	Strong		4A E12.5

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Strong		4A E13.5

Structure	Level	Pattern	Image	Note
TS23: ventricle cardiac muscle	Present		4A E15.5

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Present		4A E16.5

Structure	Level	Pattern	Image	Note
TS25: ventricle cardiac muscle	Present		4A E17.5

Structure	Level	Pattern	Image	Note
TS17: liver	Present		5A E10.5

Structure	Level	Pattern	Image	Note
TS19: liver	Present		5A E11.5

Structure	Level	Pattern	Image	Note
TS20: liver	Strong		5A E12.5

Structure	Level	Pattern	Image	Note
TS21: liver	Present		5A E13.5

Structure	Level	Pattern	Image	Note
TS22: liver	Present		5A E14.5

Structure	Level	Pattern	Image	Note
TS23: liver	Present		5A E15.5

Structure	Level	Pattern	Image	Note
TS25: liver	Strong		5A E17.5

Structure	Level	Pattern	Image	Note
TS20: lung	Strong	Regionally restricted	6A E12.5	High intensity staining was detected in the bronchioles.

Structure	Level	Pattern	Image	Note
TS21: lung	Strong	Regionally restricted	6A E13.5	High intensity staining was detected in the bronchioles.

Structure	Level	Pattern	Image	Note
TS22: lung	Strong	Regionally restricted	6A E14.5	High intensity staining was detected in the bronchioles.

Structure	Level	Pattern	Image	Note
TS23: lung	Trace	Regionally restricted	6A E15.5	Staining in the lung bronchioles was almost undetectable.

Structure	Level	Pattern	Image	Note
TS24: bronchiole	Trace		6A E16.5

Structure	Level	Pattern	Image	Note
TS25: bronchiole	Trace		6A E17.5

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Strong		8_Testis
TS28: spermatocyte		Strong		8_Testis
TS28: spermatogonium		Strong		8_Testis
TS28: Sertoli cell	Sertoli cell	Strong		8_Testis

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Weak		8_Testis inset

Structure	Level	Pattern	Image	Note
TS28: prostate epithelium	Present		8_Prostate
TS28: prostate stroma	Absent		8_Prostate

Structure	Level	Pattern	Image	Note
TS28: epithelium of epididymis	Strong	Regionally restricted	8_Epididymis	Expression was detected in the columnar epithelial cells.
TS28: muscle layer of epididymis	Absent		8_Epididymis

Structure	Level	Pattern	Image	Note
TS28: epithelium of seminal vesicle	Present	Regionally restricted	8_Seminal Vesicles	Expression was detected in the columnar epithelial cells.
TS28: muscle of seminal vesicle	Absent		8_Seminal Vesicles

Structure	Level	Pattern	Image	Note
TS28: oocyte	Strong		8_Ovary
TS28: ovary theca	Strong		8_Ovary
TS28: ovary follicle	Strong	Regionally restricted	8_Ovary	Expression was detected in the ovarian granulosa cells.
TS28: mesenchymal stroma of ovary	Absent		8_Ovary

Structure	Level	Pattern	Image	Note
TS28: corpus luteum	Strong

Structure	Level	Pattern	Image	Note
TS28: endometrium	Strong	Uniform	8_Uterus
TS28: uterine gland	Strong		8_Uterus
TS28: myometrium	Absent		8_Uterus

Structure	Level	Pattern	Image	Note
TS28: cardiac muscle tissue	Present		8_Heart	Staining in the adult was less intense than during development.

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Present		8_Lung
TS28: bronchiole epithelium	Present		8_Lung
TS28: bronchiole smooth muscle	Absent		8_Lung

Structure	Level	Pattern	Image	Note
TS28: liver	Weak		8_Liver

Structure	Level	Pattern	Image	Note
TS28: spleen	Strong		8_Spleen
TS28: spleen red pulp	Strong		8_Spleen
TS28: spleen white pulp	Strong		8_Spleen

Structure	Level	Pattern	Image	Note
TS28: renal distal tubule	Strong		8_Kidney
TS28: renal proximal tubule	Present		8_Kidney

Structure	Level	Pattern	Image	Note
TS28: skeletal muscle	Present		8_Skeletal Muscle	Expression was detected in skeletal muscle fibers, whereas the nuclei at the periphery of the fibers were negative.