Gene Expression Data
Assay Details
Assay
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Reference:
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J:206208
Makrygiannis AK, et al., Segmental and restricted localization pattern of Fras1 in the developing meningeal basement membrane in mouse. Histochem Cell Biol. 2013 Nov;140(5):595-601
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5605052
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Gene symbol:
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Fras1
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Gene name:
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Fraser extracellular matrix complex subunit 1
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Antibody:
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anti-Fras1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
1a:
embryonic day 14.5
(more )
Specimen
1a:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon meninges |
Present |
Regionally restricted |
1a
|
Expression is in the basement membrane of the lateral ventricle choroid plexus and the hem.
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Specimen
1b:
embryonic day 14.5
(more )
Specimen
1b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon meninges |
Present |
Regionally restricted |
1b
|
The protein lines the basement membrane of the septum.
|
TS22: diencephalon meninges |
Present |
Regionally restricted |
1b
|
The protein lines the meningeal basement membrane of the lamina terminalis.
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Specimen
1c:
embryonic day 13.5
(more )
Specimen
1c:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: meninges |
Present |
Regionally restricted |
1c
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The protein lines the basement membrane of the preoptic area.
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Specimen
1d:
embryonic day 13.5
(more )
Specimen
1d:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: midbrain meninges |
Present |
Regionally restricted |
1d
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The protein lines the basement membrane of the dorsal midbrain.
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Specimen
1e:
embryonic day 12.5
(more )
Specimen
1e:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: eye |
Present |
Regionally restricted |
1e
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The protein lines the basement membrane of the eye and optic stalk.
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TS20: optic stalk |
Present |
Regionally restricted |
1e
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The protein lines the basement membrane of the eye and optic stalk.
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Specimen
1f:
embryonic day 13.5
(more )
Specimen
1f:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain meninges |
Present |
Regionally restricted |
1f
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The protein lines the basement membrane of the ventral midbrain.
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TS22: hindbrain meninges |
Present |
Regionally restricted |
1f
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The protein lines the basement membrane of the ventral hindbrain and the 4th ventricle choroid plexus.
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Specimen
2b:
embryonic day 13.5
(more )
Note: (c) is a higher magnification of the boxed area in (b).
Specimen
2b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
(c) is a higher magnification of the boxed area in (b).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon meninges |
Present |
Regionally restricted |
2b
,
2c
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Expression is in the basement membrane of the lateral ventricle choroid plexus. Also at the hem of the telencephalon and is gradually reduced close to the hem and cortex boundary.
|
TS21: diencephalon meninges |
Present |
Regionally restricted |
2c
,
2b
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Expression is in the basement membrane overlying the roof of the diencephalon.
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TS21: meninges |
Weak |
Regionally restricted |
2b
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Expression is in a dotted pattern in the basement membrane of the hippocampal primordium and in a dotted pattern in the basement membrane of the dorsomedial cortex.
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TS21: brain |
Present |
Regionally restricted |
2b
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Absent in neural tissue.
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Specimen
3b:
embryonic day 14.5
(more )
Specimen
3b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon meninges |
Present |
Regionally restricted |
3b
|
The protein lines the basement membrane of the septum.
|
TS22: meninges |
Present |
Regionally restricted |
3b
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The protein lines the basement membrane of the medial prefrontal cortex.
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TS22: diencephalon meninges |
Present |
Regionally restricted |
3b
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The protein lines the meningeal basement membrane of the lamina terminalis. roof of the diencephalon.
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Specimen
3d:
embryonic day 14.5
(more )
Specimen
3d:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon meninges |
Present |
Regionally restricted |
3d
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Expression is in the basement membranes of the roof of the diencephalon, the rostro-lateral preoptic area, the ventro-posterior hypothalamus, and the third ventricle choroid plexus.
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Specimen
3f:
embryonic day 14.5
(more )
Specimen
3f:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon meninges |
Present |
Regionally restricted |
3f
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Expression is in basement membranes of the roof plate and floor plate of the diencephalon.
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