Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:210139
Hatfield JT, et al., Retinol-binding protein 4 is expressed in chondrocytes of developing mouse long bones: implications for a local role in formation of the secondary ossification center. Histochem Cell Biol. 2013 May;139(5):727-34
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5605080
|
|
Gene symbol:
|
Rbp4
|
|
Gene name:
|
retinol binding protein 4, plasma
|
|
Antibody:
|
anti-RBP4 (A0040)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
|
Results
|
Specimen
not shown E17.5:
embryonic day 17.5
(more  )
Specimen
not shown E17.5:
(close  )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
1b:
embryonic day 18.5
(more )
Note: (g) is a higher magnification of the area marked "p" in (b).
Specimen
1b:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
(g) is a higher magnification of the area marked "p" in (b).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
1b
,
1g
|
Expression is in chondrocytes involved in primary and secondary ossification and in the perichondral layer and epiphysis. Weak in chondrocytes of the proliferative zone.
|
Specimen
1c:
embryonic day 18.5
(more )
Specimen
1c:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
1c
|
Expression is in the perichondrium.
|
Specimen
1e:
embryonic day 18.5
(more )
Note: (f) is a higher magnification of (e).
Specimen
1e:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
(f) is a higher magnification of (e).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
1f
,
1e
|
Expression is in the epiphysis.
|
Specimen
2d:
postnatal day 3
(more )
Specimen
2d:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tibia |
Present |
Regionally restricted |
2d
|
Expression is in the proximal tibial epiphyses.
|
Specimen
2g:
postnatal day 7
(more )
Specimen
2g:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
2g
|
Exression is in a layer of cells below the invading canal in the proximal tibial epiphyses, in resting zone cells and pre-hypertrophic chondrocytes between the presumptive centers of primary and secondary ossification.
|
Specimen
2j:
postnatal day 14
(more )
Specimen
2j:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
2j
|
Exression is in a layer of cells below the invading canal in the proximal tibial epiphyses.
|
Specimen
2o:
postnatal day 3
(more )
Specimen
2o:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tibia |
Present |
Regionally restricted |
2o
|
Expression is in the center of the distal tibial epiphyses in perichondrial cells.
|
| TS27: foot |
Present |
Regionally restricted |
2o
|
Expression is in the proximal perichondrium of the talus.
|
Specimen
2p:
postnatal day 7
(more )
Specimen
2p:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
2p
|
Expression is in cells bordering the leading edge of ossification in the distal tibial epiphyses.
|
Specimen
2q:
postnatal day 14
(more )
Specimen
2q:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
2q
|
Expression is in the perichondrial layer facing the leading edge of ossification in the distal tibial epiphyses. Absent in perichondral cells on the articular surface and those facing the osteoid zone.
|
Specimen
3b:
postnatal day 14
(more )
Note: (c) is a higher magnification of (b).
Specimen
3b:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Note:
|
(c) is a higher magnification of (b).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
3c
,
3b
|
Expression is in the proximal tibial region between the center of secondary ossification and the perichondral layer abutting the knee joint surface.
|
Specimen
4a:
embryonic day 18.5
(more )
Specimen
4a:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
4a
|
Expression is in hypertrophic chondrocytes in the central epiphyseal region of the tibia.
|
Specimen
4b:
embryonic day 18.5
(more )
Specimen
4b:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
4b
|
Expression is in hypertrophic chondrocytes in the central epiphyseal region of the tibia.
|
Specimen
4c:
postnatal day 3
(more )
Specimen
4c:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 3
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tibia |
Present |
Regionally restricted |
4c
|
Expression is in the proliferative zone in the epiphyseal region of the tibia.
|
Specimen
4d:
postnatal day 14
(more )
Specimen
4d:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
4d
|
Expression is in the perichondrium.
|
Specimen
4e:
postnatal day 14
(more )
Specimen
4e:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 14
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
4e
|
Expression is in the resting zone.
|
Specimen
S3c:
postnatal day 1
(more )
Specimen
S3c:
(close )
|
Genetic Background:
|
C57BL/6
|
|
Age:
|
postnatal day 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
|
Analysis Tools