Gene Expression Data
Assay Details
Assay
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Reference:
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J:119509
Petrou P, et al., Overlapping and divergent localization of Frem1 and Fras1 and its functional implications during mouse embryonic development. Exp Cell Res. 2007 Mar 10;313(5):910-20
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5609957
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Gene symbol:
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Frem1
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Gene name:
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Fras1 related extracellular matrix protein 1
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Antibody:
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anti-Frem1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1A:
embryonic day 10.5
(more )
Specimen
1A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: surface ectoderm |
Present |
Regionally restricted |
1A
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Authors report expression at the dermal-epidermal interface in the subepithelial matrix of the single-layered epidermis.
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: epidermis |
Present |
Regionally restricted |
1B
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Expression at the subepithelial matrix of the two-layered epidermis.
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis |
Present |
Regionally restricted |
1C
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Expression at the subepithelial matrix of the three-layered epidermis.
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Specimen
2A:
embryonic day 15.5
(more )
Specimen
2A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: epidermis |
Present |
Regionally restricted |
2A
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Expression in the basement membrane and periderm cells in the outermost layer of the epidermis.
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Specimen
2C:
embryonic day 16.5
(more )
Specimen
2C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: epidermis |
Present |
Non-Uniform |
2C
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Marked enhancement of expression in periderm cells. Expression is non-uniform and peaks at E16. Perinuclear localization of expression in basal keratinocytes.
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Specimen
2E:
embryonic day 18.5
(more )
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: epidermis |
Present |
Regionally restricted |
2E
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Expression in basement membrane, basal keratinocytes and keratinizing epidermal cells. Perinuclear localization of expression in basal keratinocytes. Expression in periderm no longer evident.
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Specimen
3A:
embryonic day 16.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: eyelid |
Present |
Regionally restricted |
3A
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Expression in basement membrane of protruding eyeleids.
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TS24: eyelid epithelium |
Present |
Regionally restricted |
3A
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Expression in rounded periderm cells along the plane of fusion between the eyelids.
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Specimen
3C:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Fras1tm1Chpk/Fras1tm1Chpk
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: eyelid |
Present |
Regionally restricted |
3C
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Expression in basement membrane is completely abolished.
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TS24: eyelid epithelium |
Present |
Regionally restricted |
3C
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Expression in periderm cells is retained in the mutant embryo.
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Specimen
4C:
embryonic day 16.5
(more )
Specimen
4C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: hair follicle |
Strong |
Regionally restricted |
4C
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Prominent expression in hair follicle basement membrane and in interacting epithelial epidermal keratinocytes and condensing mesenchymal cells.
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Specimen
4G:
embryonic day 15.5
(more )
Specimen
4G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
embryonic day 14.5
(more )
Specimen
5B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: lung epithelium |
Present |
Regionally restricted |
5B
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Expression in basement membrane of respiratory epithelia.
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TS22: visceral pleura |
Present |
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5B
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Expression in mesothelial layer of visceral pleura.
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Specimen
5F:
embryonic day 14.5
(more )
Specimen
5F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: metanephros epithelium |
Present |
Regionally restricted |
5F
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Expression in epithelial basememnt membranes.
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Specimen
5J:
embryonic day 14.5
(more )
Specimen
5J:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: intestine epithelium |
Present |
Regionally restricted |
5J
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Expression in epithelial basement membranes.
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TS22: gut mesentery |
Present |
Regionally restricted |
5J
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Expression in intestinal mesothelium.
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