Results
|
Specimen
1a,b:
embryonic day 9.5
(more )
Note: Specimen was from the atrioventricular and conotruncal levels.
Specimen
1a,b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was from the atrioventricular and conotruncal levels.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: cardiac muscle tissue |
Absent |
|
1a
|
|
TS15: endocardial cushion |
Present |
Regionally restricted |
1b
,
1a
|
Expression was detected in most of the prismatic or cubic cells in the endocardium of the atrioventricular cushions. The endocardial cells were uniformly labeled.
|
Specimen
1c,d,e:
embryonic day 9.5
(more )
Note: Specimen was from the levels of atrioventricular canal and conotruncus (more cranial than in 1a).
Specimen
1c,d,e:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was from the levels of atrioventricular canal and conotruncus (more cranial than in 1a).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: cardiac muscle tissue |
Absent |
|
1d
,
1e
,
1c
|
|
TS15: endocardial cushion |
Present |
Regionally restricted |
1d
,
1e
,
1c
|
Expression was detected in most of the prismatic or cubic cells in the endocardium of the atrioventricular cushions. The endocardial cells were uniformly labeled.
|
Specimen
1f:
embryonic day 9.5
(more )
Note: Specimen was from the distal level of the conotruncus.
Specimen
1f:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was from the distal level of the conotruncus.
|
Specimen
2a,b:
embryonic day 10.5
(more )
Note: Specimen was from the atrioventricular level.
Specimen
2a,b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was from the atrioventricular level.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: heart |
Present |
Regionally restricted |
2b
,
2a
|
Expression was detected in atrioventricular cushion mesenchymal cells.
|
TS17: cardiac muscle tissue |
Absent |
|
2a
,
2b
|
|
TS17: endocardial lining |
Present |
Regionally restricted |
2a
,
2b
|
About 15% of cubic endocardial cells had positive signal.
|
Specimen
2c,d,e:
embryonic day 10.5
(more )
Specimen
2c,d,e:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: bulbus cordis rostral half |
Present |
Regionally restricted |
2e
,
2d
,
2c
|
An average of 17% of labeled endothelial cells are found, usually prismatic or cubic. Staining density in mesenchymal cells in the proximal part was higher than in the distal part.
|
Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: endocardial lining |
Present |
Regionally restricted |
|
An average of 8% of labeled cells was present in the endocardium.
|
Specimen
3a,b,c:
embryonic day 12.5
(more )
Note: Specimen was from the level of one atrioventricular cushion and two ventricles.
Specimen
3a,b,c:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was from the level of one atrioventricular cushion and two ventricles.
|
Specimen
3d:
embryonic day 12.5
(more )
Specimen
3d:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3e,f,g:
embryonic day 12.5
(more )
Specimen
3e,f,g:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: heart left ventricle |
Present |
Regionally restricted |
3e
,
3g
|
Expression was detected in cells in the anterior wall of the left ventricle. Staining was not seen in the posterior of the left ventricle.
|
TS20: bulbar ridge |
Present |
Regionally restricted |
3e
,
3f
|
Expression was detected in the mesenchyme of the conotruncal ridges. In the conotruncus, the repartition of positive cells seems regular, with possibly a higher proportion in the distal part.
|
TS20: interventricular septum |
Absent |
|
3e
|
|
TS20: right ventricle cardiac muscle |
Absent |
|
3e
|
|
Specimen
3h:
embryonic day 12.5
(more )
Specimen
3h:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: bulbar ridge |
Present |
Regionally restricted |
3h
|
Expression was detected in the mesenchyme of the conotruncal ridges.
|
Specimen
not shown E13.5:
embryonic day 13.5
(more )
Specimen
not shown E13.5:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: left ventricle cardiac muscle |
Present |
Regionally restricted |
|
In this section, staining was in a continuous strip at the level of the apex.
|
Specimen
4a,b:
embryonic day 13.5
(more )
Specimen
4a,b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4c,d,e:
embryonic day 13.5
(more )
Specimen
4c,d,e:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: atrioventricular endocardial cushion |
Present |
Regionally restricted |
4c
,
4d
|
Expression was in the mesenchyme.
|
TS21: bulbar ridge |
Present |
Regionally restricted |
4c
,
4d
|
Expression was in the mesenchyme.
|
TS21: left ventricle cardiac muscle |
Present |
Regionally restricted |
4c
,
4e
|
Expression was detected in the anterior and left lateral wall of the left ventricle. In this section, label was seen as a continuous strip of cells toward the top of the ventricle. Label was only in the compact layer.
|
Specimen
4f:
embryonic day 13.5
(more )
Note: Section was distal to that in 4c
Specimen
4f:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Section was distal to that in 4c
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: left ventricle cardiac muscle |
Present |
Regionally restricted |
4f
|
Expression was detected in the anterior and left lateral wall of the left ventricle. In this section, label was seen as a continuous strip of cells toward the top of the ventricle. Label was only in the compact layer.
|
TS21: bulbar ridge |
Present |
Regionally restricted |
4f
|
Expression was in the mesenchyme of the conotrucal ridges.
|
Specimen
4g:
embryonic day 13.5
(more )
Note: Section was distal to that in 4c
Specimen
4g:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Section was distal to that in 4c
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: bulbar ridge |
Present |
Regionally restricted |
4g
|
Expression was in the mesenchyme of the conotruncal ridges.
|
Specimen
4h:
embryonic day 13.5
(more )
Note: Section was distal to that in 4c
Specimen
4h:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Section was distal to that in 4c
|
Specimen
not shown E14.5:
embryonic day 14.5
(more )
Specimen
not shown E14.5:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: left ventricle cardiac muscle |
Present |
Regionally restricted |
|
Expression looked both continuous and discontinuous. Continuous at the level of the anterior wall. Discontinuous at the level of the lateral wall of the left ventricle. The thickness appeared variable.
|
TS22: aortic valve |
Present |
Single cells |
|
Rare immunostained cells were observed.
|
TS22: pulmonary valve |
Present |
Single cells |
|
Rare immunostained cells were observed.
|
Specimen
not shown E15.5:
embryonic day 15.5
(more )
Specimen
not shown E15.5:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: left ventricle cardiac muscle |
Present |
Regionally restricted |
|
The thickness of the labelled strip has decreased as well as its extension to the left.
|
Specimen
not shown E17.5:
embryonic day 17.5
(more )
Specimen
not shown E17.5:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: interventricular septum |
Present |
Regionally restricted |
|
Expression was in the endothelium of some vessels.
|
Specimen
5a,b:
embryonic day 19.5
(more )
Specimen
5a,b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 19.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|