Gene Expression Data
Assay Details
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Assay
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Reference:
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J:106421
Komine Y, et al., Novel transcription factor zfh-5 is negatively regulated by its own antisense RNA in mouse brain. Mol Cell Neurosci. 2006 Feb;31(2):273-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5632131
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Gene symbol:
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Zfhx2
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Gene name:
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zinc finger homeobox 2
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Probe:
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zfh-5 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The authors did not specify which probe was used. Either this probe or zfh-5 cDNA2 MGI:5632099, zfh-5 cDNA3 MGI:5632100, or zfh-5 cDNA4 MGI:5632101 were used.
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Results
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Specimen
1B:
embryonic day 13.5
(more  )
Specimen
1B:
(close  )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A E16.5:
embryonic day 16.5
(more )
Specimen
2A E16.5:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A P2:
postnatal day 2
(more )
Specimen
2A P2:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A P7:
postnatal day 7
(more )
Specimen
2A P7:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2A P20:
postnatal day 20
(more )
Specimen
2A P20:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal day 20
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2B:
postnatal day 7
(more )
Specimen
2B:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C left:
postnatal adult
(more )
Specimen
2C left:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C right:
postnatal adult
(more )
Specimen
2C right:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2D a:
postnatal adult
(more )
Specimen
2D a:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
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2D aS
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Specimen
2D b:
postnatal adult
(more )
Specimen
2D b:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
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2D bS
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Specimen
2D c:
postnatal adult
(more )
Specimen
2D c:
(close )
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Genetic Background:
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either: C57BL/6Cr or C57BL/6J or ICR
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A +/+ left:
embryonic day 16.5
(more )
Specimen
5A +/+ left:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A +/+ right:
embryonic day 16.5
(more )
Specimen
5A +/+ right:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Present |
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5A Sense right panel +/+
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| TS24: pons |
Absent |
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5A Sense right panel +/+
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Absent in pontine nuclei.
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Specimen
5A deltaASC/deltaASC left:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zfhx2ostm1Ymri/Zfhx2ostm1Ymri
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Strong |
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5A Sense mutant
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Expression increased in various areas of the brain compared to wild-type.
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| TS24: cortical plate |
Strong |
Uniform |
5A c
,
5A Sense mutant
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Specimen
5A deltaASC/deltaASC right:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zfhx2ostm1Ymri/Zfhx2ostm1Ymri
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brain |
Strong |
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5A Sense right panel mutant
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Expression increased in various areas of the brain compared to wild-type.
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| TS24: pons |
Present |
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5A Sense right panel mutant
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Ectopic expression in pontine nuclei.
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Specimen
5B +/+:
postnatal day 8
(more )
Specimen
5B +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B mutant:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Zfhx2ostm1Ymri/Zfhx2ostm1Ymri
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools