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Gene Expression Data
Assay Details
Assay
Reference: J:207604 Duffie R, et al., The Gpr1/Zdbf2 locus provides new paradigms for transient and dynamic genomic imprinting in mammals. Genes Dev. 2014 Mar 1;28(5):463-78
Assay type: RT-PCR
MGI Accession ID: MGI:5634787
Gene symbol: Zdbf2
Gene name: zinc finger, DBF-type containing 2
Probe: Liz-pC, Liz-pD
Assay notes: RT-PCR pyrosequencing to determine allelic ratio.
Results
Image: 3C left ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
gDNA Control
WT E3.5 TS4: blastocyst Present Not Specified; poly-A+ RNA involves: C57BL/6J * CAST/Ei Not Specified Paternal.
WT E3.5 TS4: blastocyst Present Not Specified; poly-A+ RNA involves: C57BL/6J * CAST/Ei Not Specified Maternal.
Dnmt3L-/+ E3.5 TS4: blastocyst Present Not Specified; poly-A+ RNA involves: 129S6/SvEvTac * C57BL/6J Dnmt3ltm1Bes/Dnmt3l+Not Specified Paternal.
Dnmt3L-/+ E3.5 TS4: blastocyst Present Not Specified; poly-A+ RNA involves: 129S6/SvEvTac * C57BL/6J Dnmt3ltm1Bes/Dnmt3l+Not Specified Maternal.
Notes:
(a) Wild type blastocysts showed paternal-specific expression. Dnmt3l-/+ blastocysts showed bi-allelic expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory