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Gene Expression Data
Assay Details
Assay
Reference: J:150047 Lei K, et al., SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice. Proc Natl Acad Sci U S A. 2009 Jun 23;106(25):10207-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5637338
Gene symbol: Syne1
Gene name: spectrin repeat containing, nuclear envelope 1
Antibody: anti-Syne-1
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 1A: embryonic day 18.5 (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS26: transversus thoracis Present 1A , 1B , 1C Expression is localized to the nuclear envelope of both synaptic and nonsynaptic nuclei.
Specimen 1D: embryonic day 18.5; Sun1tm1Mhan/Sun1tm1Mhan (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS26: transversus thoracis Present 1D Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
Specimen 1E: embryonic day 18.5; Sun1tm1Mhan/Sun1tm1Mhan (more )
Structure Level Pattern Image Note
TS26: transversus thoracis Present 1E Expression is localized to the nuclear envelope of nonsynaptic nuclei; the distribution of these nuclei in these mice appears similar to wild type.
Specimen 2C WT: postnatal adult (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS28: tibialis anterior Present 2C upper left Expression is localized to the nuclear envelope of synaptic nuclei.
Specimen 2C +/-; +/-: postnatal adult; Sun1tm1Mhan/Sun1+, Sun2tm1Mhan/Sun2+ (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS28: tibialis anterior Present 2C upper right Expression is localized to the nuclear envelope of synaptic nuclei; the distribution of these nuclei in these mice appears similar to wild type.
Specimen 2C -/-; +/-: postnatal adult; Sun1tm1Mhan/Sun1tm1Mhan, Sun2tm1Mhan/Sun2+ (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS28: tibialis anterior Present 2C lower left Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
Specimen 2C +/-; -/-: postnatal adult; Sun1tm1Mhan/Sun1+, Sun2tm1Mhan/Sun2tm1Mhan (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS28: tibialis anterior Present 2C lower right Expression is localized to the nuclear envelope of synaptic nuclei; the number of these nuclei is reduced in the mutant compared to wild type.
Specimen 5A: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 5C , 5A Expression is localized in the nuclear envelope.
Specimen 5D: embryonic day 18.5; Sun1tm1Mhan/Sun1+, Sun2tm1Mhan/Sun2+ (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 5D , 5F Expression is localized in the nuclear envelope; no differences in localization between these mice and wild type were observed.
Specimen 5G: embryonic day 18.5; Sun1tm1Mhan/Sun1tm1Mhan, Sun2tm1Mhan/Sun2+ (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 5I , 5G Expression is localized in the nuclear envelope; however, the amount of localization is reduced compared to that seen in wild type mice.
Specimen 5J: embryonic day 18.5; Sun1tm1Mhan/Sun1+, Sun2tm1Mhan/Sun2tm1Mhan (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Present 5L , 5J Expression is localized in the nuclear envelope; however, the amount of localization is reduced compared to that seen in wild type mice.
Specimen 5M: embryonic day 18.5; Sun1tm1Mhan/Sun1tm1Mhan, Sun2tm1Mhan/Sun2tm1Mhan (more )
Structure Level Pattern Image Note
TS26: intercostal muscle Ambiguous 5M , 5O No localization to the nuclear envelope is seen in the double mutant.
Specimen S1F: postnatal adult (more )
Note: The tissue is also stained with alpha-bungrotoxin (red) to label the neuromuscular junction.
Structure Level Pattern Image Note
TS28: skeletal muscle Present S1F Expression is present in nuclei at the neuromuscular junction.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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