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Col1a1tm1(Sry)Aqui
Targeted Allele Detail
Summary
Symbol: Col1a1tm1(Sry)Aqui
Name: collagen, type I, alpha 1; targeted mutation 1, Alexander Quinn
MGI ID: MGI:5638900
Synonyms: Sry-L741
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm1(Sry)Aqui page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:215196
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence)
Mutation:    Insertion
 
Col1a1tm1(Sry)Aqui expresses 1 gene
 
Mutation details: RMCE on KH2 ES cells introduced into the 3' UTR the Sry coding region with 8031 bp of the upstream flanking inverted repeat and downstream inverted repeat including the StuI restriction site followed by an ampillicin resistance gene, PGK promoter, hygromycin resistance gene and polyadenylation sequence. FRT sites flank the entire insertion. (J:215196)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  163 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:215196 Quinn A, et al., A site-specific, single-copy transgenesis strategy to identify 5' regulatory sequences of the mouse testis-determining gene Sry. PLoS One. 2014;9(4):e94813
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory