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Col1a1tm1(tetO-GFP/RNAi:Sirt1)Pyc
Targeted Allele Detail
Summary
Symbol: Col1a1tm1(tetO-GFP/RNAi:Sirt1)Pyc
Name: collagen, type I, alpha 1; targeted mutation 1, Peter Y Chuang
MGI ID: MGI:5646012
Synonyms: Col-TGM, Sirt1RNAi
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm1(tetO-GFP/RNAi:Sirt1)Pyc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:213867
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Knockdown, RMCE-ready, Reporter)
Mutation:    Insertion
  Col1a1tm1(tetO-GFP/RNAi:Sirt1)Pyc involves 1 genes/genome features (Sirt1) View all
 
Mutation details: RMCE on KH2 cells inserted a cassette that contains the tetracycline promoter and a GFP fusion with a Mir30e-based short hairpin sequence targeting Sirt1 (Sirt1.688) into the 3'UTR. (J:213867)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  163 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:213867 Chuang PY, et al., In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells. Am J Pathol. 2014 Jul;184(7):1940-56
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
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