Pip<tm1.1(cre/ERT2)Ovi> Targeted Allele Detail MGI Mouse (MGI:5661584)

Pip^{tm1.1(cre/ERT2)Ovi}
Targeted Allele Detail

Summary

Symbol:	Pip^{tm1.1(cre/ERT2)Ovi}
Name:	prolactin induced protein; targeted mutation 1.1, Catherine E Ovitt
MGI ID:	MGI:5661584
Synonyms:	Pip ^GCE, Pip-GFP-CreERT2, Pip^tm2(GCE)Ovi
Gene:	Pip Location: Chr6:41824483-41828999 bp, + strand Genetic Position: Chr6, 20.05 cM, cytoband B2
Alliance:	Pip^{tm1.1(cre/ERT2)Ovi} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:229520
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Mutation
description

Allele Type:

Targeted (Inducible, Recombinase)

Inducer:

tamoxifen

Mutations:

Insertion, Intragenic deletion

Pip^{tm1.1(cre/ERT2)Ovi} expression driven by 1 gene

Mutation details: The eGFP-CreERT2 (GCE) fragment with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of the endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. (J:229520)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pip (mouse) Summary of all recombinase alleles driven by Pip. Comparative matrix view of recombinase activities Alliance mouse Pip gene page

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Pip Mutation:	16 strains or lines available

References

Original:	J:229520 Maruyama EO, et al., Cell-Specific Cre Strains For Genetic Manipulation in Salivary Glands. PLoS One. 2016;11(1):e0146711
All:	2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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