Probe:	Irx3 cDNA8
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS8: extraembryonic component	Present
TS8: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present			Expression is present in the neural plate.

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present		1B Irx3
TS20: heart	Present		1B Irx3
TS20: facial ganglion	Present		1B Irx3
TS20: vestibulo-cochlear ganglion	Present		1B Irx3
TS20: metencephalon	Present		1B Irx3
TS20: spinal cord	Present		1B Irx3	Expression is present in the neural tube.
TS20: otic capsule	Present		1B Irx3
TS20: skin	Present		1B Irx3

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2D	Expression is present ventrolaterally.
TS19: metencephalon	Present	Regionally restricted	2D	Expression is present dorsolaterally.
TS19: metencephalon basal plate	Strong	Regionally restricted	2D	Expression is found in the sensory region and lateral region of the motor part of the pons.
TS19: cerebellum primordium	Weak		2D

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		2J
TS19: neural tube alar columns	Present		2J	Expression is in the mantle layer.
TS19: neural tube basal columns	Present		2J	Expression is in the mantle layer.
TS19: neural tube lateral wall	Present		2J	Expression is present in motor neurons.
TS19: neural tube floor plate	Absent		2J
TS19: neural tube roof plate	Absent		2J
TS19: notochord	Present		2J

Structure	Level	Pattern	Image	Note
TS19: inner ear	Present		2P	Expression is present in the otic vesicle.
TS19: facial ganglion	Present	Regionally restricted	2P	Expression is present in the ganglia medial to the cranial cardinal vein.

Structure	Level	Pattern	Image	Note
TS21: neural retinal epithelium	Present	Regionally restricted	3G	Expression is present in the marginal zone.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar	Present		3K
TS21: upper jaw molar	Present		3K
TS21: oral epithelium	Present		3K	Expression extends lateral from the molar tooth primordia.

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Present	Regionally restricted		Expression is present in the proximal region over the antero-posterior axis, highest at the dorsal side.

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present	Regionally restricted		Expression is present proximally.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present	Regionally restricted		Expression is present in the proximal limb.
TS19: hindlimb bud mesenchyme	Present	Regionally restricted		Expression is present in the proximal limb.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted		Expression is present in the proximal limb.
TS20: hindlimb mesenchyme	Present	Regionally restricted		Expression is present in the proximal limb.

Structure	Level	Pattern	Image	Note
TS21: forelimb interdigital region mesenchyme	Present
TS21: hindlimb interdigital region mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS22: forelimb interdigital region mesenchyme	Present		4E

Structure	Level	Pattern	Image	Note
TS22: hindlimb interdigital region mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	4F	Expression is present in the proximal region.

Structure	Level	Pattern	Image	Note
TS19: trachea epithelium	Present		5D	Expression in the laryncho-tracheal groove endoderm.
TS19: trachea mesenchyme	Absent		5D	No expression in the laryncho-tracheal groove mesenchyme.
TS19: foregut epithelium	Present		5D	Expression in foregut endoderm.

Structure	Level	Pattern	Image	Note
TS21: left ventricle cardiac muscle	Present		5J	Expression is present in the trabeculated myocardium.
TS21: right ventricle cardiac muscle	Present		5J	Expression is present in the trabeculated myocardium.
TS21: lung epithelium	Present		5J
TS21: lung mesenchyme	Absent		5J

Structure	Level	Pattern	Image	Note
TS21: gonad	Strong		5M
TS21: metanephros	Present	Regionally restricted	5M	Expression is present in the margin between the cortex and the medulla.
TS21: cap mesenchyme	Present	Regionally restricted	5M	Expression is present in the blastema.
TS21: stomach	Present	Regionally restricted	5M	Expression is present in outer curvature of the stomach endoderm.

Structure	Level	Pattern	Image	Note
TS14: primitive ventricle cardiac muscle	Present	Regionally restricted		Expression is present in the trabeculations.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present	Regionally restricted		Expression is medial, adjacent to the hindbrain.

Structure	Level	Pattern	Image	Note
TS17: lung epithelium	Present
TS17: lung mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Absent
TS19: gonad	Strong
TS19: acoustic ganglion	Present			Expression is present in the complex.
TS19: facial ganglion	Present			Expression is present in the complex.

Structure	Level	Pattern	Image	Note
TS21: nasal capsule	Present			Expression is present in the cartilage.
TS21: dermis	Present
TS21: epidermis	Weak
TS21: mammary gland primordium	Present
TS21: vibrissa follicle	Weak			Expression is present in the germinal matrix.
TS21: thalamus	Present