Probe:	Rorbeta cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS25: thalamus	Present	Regionally restricted	1D	Low expression in the ventroposterior, posterior and mediodorsal nuclei, and moderate expression in the medial geniculate nucleus. No expression in the dorsal lateral geniculate nucleus. High expression in the centromedial nuclei.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Present	Regionally restricted	1E	Low expression in the ventroposterior, posterior and mediodorsal nuclei, and moderate expression in the medial geniculate nucleus. No expression in the dorsal lateral geniculate nucleus. High expression in the centromedial nuclei.

Structure	Level	Pattern	Image	Note
TS27: ventral posterior thalamic group	Present	Graded	1F	Increased expression compared to E17.5. Expressed strongly in what appears to be the medial subdivision that relays trigeminal system inputs, and low or nondetectable expression in the lateral subdivision that relays lemniscal inputs.
TS27: medial dorsal thalamic nucleus	Present		1F
TS27: ventral lateral geniculate nucleus	Present		1F
TS27: dorsal lateral geniculate nucleus	Weak		1F	Very low expression.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Absent		2B

Structure	Level	Pattern	Image	Note
TS21: thalamus mantle layer	Absent		2E

Structure	Level	Pattern	Image	Note
TS20: neocortex	Absent		3A

Structure	Level	Pattern	Image	Note
TS22: neocortex	Present	Regionally restricted	3B	Expressed in the emerging cortical plate in the lateral part of neocortex at a rostral level.
TS22: ganglionic eminence	Present		3B

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present	Regionally restricted	3C	Expressed in cortical plate. Expression is much higher at rostral levels than at caudal levels. High lateral to low medial gradient.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present	Regionally restricted	3D	Expressed in cortical plate. Expression is much higher at rostral levels than at caudal levels.

Structure	Level	Pattern	Image	Note
TS24: neocortex	Present	Graded	3E	High rostral to low caudal gradient.

Structure	Level	Pattern	Image	Note
TS26: neocortex	Present	Regionally restricted	3F	Higher expression in the putative somatosensory area, located roughly in the middle of mediolateral axis. Expression is much higher at rostral levels than at caudal levels.

Structure	Level	Pattern	Image	Note
TS26: neocortex	Weak	Uniform	3G	Expression increased selectively in the presumptive visual and auditory areas. Expression is much higher at rostral levels than at caudal levels.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Strong	Regionally restricted	4A	More pronounced expression in the somatosensory area than at E18.5. The level of expression is strongest in the middle layer.
TS27: primary somatosensory cortex	Present		4A

Structure	Level	Pattern	Image	Note
TS27: neocortex	Strong	Regionally restricted	4E	Highly expressed in the putative visual area in the caudal neocortex. The level of expression is strongest in the middle layer.

Structure	Level	Pattern	Image	Note
TS27: cortical layer IV	Strong	Regionally restricted	4B	Expressed in the primary somatosensory, visual, and auditory areas.
TS27: cortical layer V	Present	Scattered	4B
TS27: primary somatosensory cortex	Strong	Regionally restricted	4B	Expressed at a high level in layer 4, and in scattered cells in layer 5.
TS27: visual cortex	Strong	Regionally restricted	4B	Expressed at a high level in layer 4, and in scattered cells in layer 5.

Structure	Level	Pattern	Image	Note
TS27: cortical layer IV	Strong	Regionally restricted	4F	Expressed in the primary somatosensory, visual, and auditory areas.
TS27: cortical layer V	Present	Scattered	4F
TS27: primary somatosensory cortex	Strong	Regionally restricted	4F	Expressed at a high level in layer 4, and in scattered cells in layer 5.
TS27: visual cortex	Strong	Regionally restricted	4F	Expressed at a high level in layer 4, and in scattered cells in layer 5.

Structure	Level	Pattern	Image	Note
TS28: primary somatosensory cortex	Strong	Regionally restricted	4C	Expressed in layers 4 and 5.
TS28: cortical layer IV	Strong	Regionally restricted	4C	Expressed in the primary somatosensory area, including the posteromedial barrel subfield.
TS28: cortical layer V	Strong	Regionally restricted	4C	Expressed in the primary somatosensory area, including the posteromedial barrel subfield.

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Strong	Regionally restricted	4G	Expressed in the primary visual area.
TS28: cortical layer V	Strong	Regionally restricted	4G	Expressed in the primary visual area.
TS28: visual cortex	Strong	Regionally restricted	4G	Expressed as a thick band in layers 4 and 5.

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present		6E

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present		6G

Structure	Level	Pattern	Image	Note
TS27: medial geniculate nucleus	Present	Graded		Increased expression compared to E17.5. Expressed strongly in what appears to be the medial subdivision that relays trigeminal system inputs, and low or nondetectable expression in the lateral subdivision that relays lemniscal inputs.

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present

Structure	Level	Pattern	Image	Note
TS27: neocortex	Strong	Regionally restricted		Highly expressed in auditory areas in the caudal neocortex.