Runx2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:220612 Holmes G, et al., BCL11B expression in intramembranous osteogenesis during murine craniofacial suture development. Gene Expr Patterns. 2015 Jan;17(1):16-25
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5699546
Gene symbol:	Runx2
Gene name:	runt related transcription factor 2

Antibody:	anti-RUNX2 (HPA022040)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1B2 ©

1B3 ©

Specimen 1 B2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: premaxilla	Present		1B2 , 1B3	Expression is in osteoprogenitors and mature osteoblasts.
TS22: face mesenchyme	Absent		1B3 , 1B2

1C2 ©

1C3 ©

Specimen 1 C2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Regionally restricted	1C3 , 1C2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: premaxilla	Present	Regionally restricted	1C3 , 1C2	Expression is in osteoprogenitors and mature osteoblasts.

1D2 ©

1D3 ©

Specimen 1 D2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		1D2 , 1D3	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		1D2 , 1D3	Expression is in osteoprogenitors and mature osteoblasts.

1E2 ©

1E3 ©

Specimen 1 E2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: mandible	Present		1E3 , 1E2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: face mesenchyme	Absent		1E3 , 1E2

1F2 ©

1F3 ©

Specimen 1 F2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: inter-parietal bone primordium	Present		1F3 , 1F2	Expression is in osteoprogenitors and mature osteoblasts.

2B2 ©

2B3 ©

Specimen 2 B2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: nasal septum	Present	Regionally restricted	2B2 , 2B3	Expression is in the vomer bones of the nasal septum.

2C2 ©

2C3 ©

Specimen 2 C2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2C3 , 2C2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		2C3 , 2C2	Expression is in osteoprogenitors and mature osteoblasts.

2D2 ©

2D3 ©

Specimen 2 D2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2D3 , 2D2	Expression is in osteoprogenitors and mature osteoblasts.
TS22: palatal shelf	Present		2D3 , 2D2	Expression is in osteoprogenitors and mature osteoblasts.

2E2 ©

2E3 ©

Specimen 2 E2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present		2E3 , 2E2	Expression is in osteoprogenitors and mature osteoblasts, absent in the anterior part.
TS22: palatal shelf	Present		2E3 , 2E2	Expression is in osteoprogenitors and mature osteoblasts, absent in the anterior part.

2F2 ©

2F3 ©

Specimen 2 F2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: nose	Present	Regionally restricted	2F3 , 2F2	Expression is in osteoprogenitors and mature osteoblasts.

2G2 ©

2G3 ©

Specimen 2 G2: embryonic day 14.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS22: frontal bone primordium	Present		2G3 , 2G2	Expression is in osteoprogenitors and mature osteoblasts. Expression is reduced at the edges.

3B2 ©

3B3 ©

Specimen 3 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: face mesenchyme	Absent		3B3 , 3B2

3C2 ©

3C3 ©

Specimen 3 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: nose	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts of the nasal bones.
TS24: frontal bone	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: premaxilla	Present	Regionally restricted	3C3 , 3C2	Expression is in osteoprogenitors and mature osteoblasts.

3D2 ©

3D3 ©

Specimen 3 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	3D2 , 3D3	Expression is in osteoprogenitors and mature osteoblasts.
TS24: premaxilla	Present	Regionally restricted	3D2 , 3D3	Expression is in osteoprogenitors and mature osteoblasts.

3E2 ©

3E3 ©

Specimen 3 E2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	3E3 , 3E2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	3E3 , 3E2	Expression is in osteoprogenitors and mature osteoblasts.

3F2 ©

3F3 ©

Specimen 3 F2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	3F3 , 3F2	Expression is in osteoprogenitors and mature osteoblasts.

4B2 ©

4B3 ©

Specimen 4 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	4B3 , 4B2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	4B3 , 4B2	Expression is in osteoprogenitors and mature osteoblasts.

4C2 ©

4C3 ©

Specimen 4 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	4C3 , 4C2	Expression is in osteoprogenitors and mature osteoblasts.

4D2 ©

4D3 ©

Specimen 4 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: secondary palate	Present	Regionally restricted	4D3 , 4D2	Expression is in osteoprogenitors and mature osteoblasts.

4E2 ©

4E3 ©

Specimen 4 E2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	4E2 , 4E3	Expression is in osteoprogenitors and mature osteoblasts.

4F2 ©

4F3 ©

Specimen 4 F2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	4F2 , 4F3	Expression is in osteoprogenitors and mature osteoblasts in the zygomatic process of maxillary bone.

4G2 ©

4G3 ©

Specimen 4 G2: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: jaw skeleton	Present	Regionally restricted	4G2 , 4G3	Expression is in osteoprogenitors and mature osteoblasts.

5A2 ©

5A3 ©

Specimen 5 A2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: parietal bone	Present	Regionally restricted	5A2 , 5A3	Expression is in osteoprogenitors and mature osteoblasts.

5B2 ©

5B3 ©

Specimen 5 B2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	5B2 , 5B3	Expression is in osteoprogenitors and mature osteoblasts.

5C2 ©

5C3 ©

Specimen 5 C2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: frontal bone	Present	Regionally restricted	5C3 , 5C2	Expression is in osteoprogenitors and mature osteoblasts.
TS24: parietal bone	Present	Regionally restricted	5C3 , 5C2	Expression is in osteoprogenitors and mature osteoblasts.

5D2 ©

5D3 ©

Specimen 5 D2: embryonic day 16.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS24: parietal bone	Present	Regionally restricted	5D3 , 5D2	Expression is in osteoprogenitors and mature osteoblasts and is weak in the squamo-parietal suture.
TS24: temporal bone	Present	Regionally restricted	5D3 , 5D2	Expression is in osteoprogenitors and mature osteoblasts and is weak in the squamo-parietal suture.

6B2 ©

6B3 ©

Specimen 6 B2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: nasal bone	Present	Regionally restricted	6B2 , 6B3	Expression is in osteoprogenitors and mature osteoblasts, and is absent at the edges.
TS26: premaxilla	Present	Regionally restricted	6B2 , 6B3	Expression is in osteoprogenitors and mature osteoblasts, and is absent at the edges.

6C2 ©

6C3 ©

Specimen 6 C2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: premaxilla	Present	Regionally restricted	6C3 , 6C2	Expression is in osteoprogenitors and mature osteoblasts.

6D2 ©

6D3 ©

Specimen 6 D2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: nasal bone	Present	Regionally restricted	6D2 , 6D3	Expression is in osteoprogenitors and mature osteoblasts.

6E2 ©

6E3 ©

Specimen 6 E2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: frontal bone	Present	Regionally restricted	6E2 , 6E3	Expression is in osteoprogenitors and mature osteoblasts.
TS26: parietal bone	Present	Regionally restricted	6E2 , 6E3	Expression is in osteoprogenitors and mature osteoblasts.

6F2 ©

6F3 ©

Specimen 6 F2: embryonic day 18.5 (more

)
Note: Double labeled: green - Runx2; red - Bcl11b (assay MGI:5699414).

Structure	Level	Pattern	Image	Note
TS26: frontal bone	Present	Regionally restricted	6F3 , 6F2	Expression is in osteoprogenitors and mature osteoblasts.

7B ©

Specimen 7B: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7B	Expression is in osteoprogenitors and mature osteoblasts in the zyogomatic process of the maxillary bone.

7D ©

Specimen 7D: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7D	Expression is in osteoprogenitors and mature osteoblasts.
TS24: secondary palate	Present	Regionally restricted	7D	Expression is in osteoprogenitors and mature osteoblasts.

7F ©

Specimen 7F: embryonic day 16.5 (more

)
Note: Double labeled with alkaline phosphatase (red).

Structure	Level	Pattern	Image	Note
TS24: maxilla	Present	Regionally restricted	7F	Expression is in osteoprogenitors and mature osteoblasts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/05/2024 MGI 6.24