Nkx2-1<tm2.1(flpo)Zjh> Targeted Allele Detail MGI Mouse (MGI:5700385)

Nkx2-1^{tm2.1(flpo)Zjh}
Targeted Allele Detail

Summary

Symbol:	Nkx2-1^{tm2.1(flpo)Zjh}
Name:	NK2 homeobox 1; targeted mutation 2.1, Z Josh Huang
MGI ID:	MGI:5700385
Synonyms:	Nkx2.1-Flp, Nkx2.1-ires-Flpo
Gene:	Nkx2-1 Location: Chr12:56578741-56583570 bp, - strand Genetic Position: Chr12, 24.42 cM, cytoband C1-C3
Alliance:	Nkx2-1^{tm2.1(flpo)Zjh} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:235123
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Recombinase)

Mutation:

Insertion

Nkx2-1^{tm2.1(flpo)Zjh} expression driven by 1 gene

Mutation details: The targeting vector is designed to insert an internal ribosome entry site (IRES) fused to flpo recombinase gene (a codon-optimized FLPe gene modified for translation in mammalian cells and higher recombination efficiency in cells and in mice), followed by a loxP-flanked neo cassette, downstream of the stop codon of the gene. Cre-mediated recombination removed the floxed neo cassette. (J:235123)

Recombinase
activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nkx2-1 (mouse) Summary of all recombinase alleles driven by Nkx2-1. Alliance mouse Nkx2-1 gene page

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Nkx2-1 Mutation:	24 strains or lines available

References

Original:	J:235123 He M, et al., Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron. 2016 Sep 21;91(6):1228-1243
All:	14 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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