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Col1a1tm1(tetO-Gata4/mCherry)Amza
Targeted Allele Detail
Summary
Symbol: Col1a1tm1(tetO-Gata4/mCherry)Amza
Name: collagen, type I, alpha 1; targeted mutation 1, Alfonso Martinez Arias
MGI ID: MGI:5700544
Synonyms: ColA1TetO-Gata4-mCherry, GATA4-mCherry
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm1(tetO-Gata4/mCherry)Amza page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:227467
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Col1a1tm1(tetO-Gata4/mCherry)Amza expresses 1 gene
 
Col1a1tm1(tetO-Gata4/mCherry)Amza expression driven by 1 gene
 
Mutation details: RMCE on KH2 cells, which contain a FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1, replaced the FRT-flanked neo with a cassette that contains the tetracycline promoter driving inducible expression of an mCherry Gata4 fusion. (J:226600, J:227467)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  163 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:227467 Schroter C, et al., FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells. Development. 2015 Dec 15;142(24):4205-16
All:  3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory