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Col1a1tm2(tetO-Ccnb2)Jvd
Targeted Allele Detail
Summary
Symbol: Col1a1tm2(tetO-Ccnb2)Jvd
Name: collagen, type I, alpha 1; targeted mutation 2, Jan M A van Deursen
MGI ID: MGI:5705291
Synonyms: Ccnb2-HA, Ccnb2T15, Ccnb2T16
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance: Col1a1tm2(tetO-Ccnb2)Jvd page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:219399
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Mutation
description
Allele Type:    Targeted (Inducible, Inserted expressed sequence)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Col1a1tm2(tetO-Ccnb2)Jvd expresses 1 gene
 
Mutation details: RMCE on KH2 cells replaced the FRT-flanked neo cassette with a cassette that contains a splice acceptor, polyadenylation sequence and the tetracycline promoter driving inducible expression of the tetracycline promoter and an HA-tagged cDNA for mouse Ccnb2, polyadenylation sequence and hygromycin resistance cassette with an FRT site. (J:219399)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  163 strains or lines available
Notes
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm3(neo/hygro*))Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
References
Original:  J:219399 Nam HJ, et al., Cyclin B2 and p53 control proper timing of centrosome separation. Nat Cell Biol. 2014 Jun;16(6):538-49
All:  1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/12/2024
MGI 6.24 		The Jackson Laboratory