Samd8tm2.1Kwi
Targeted Allele Detail
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| Symbol: |
Samd8tm2.1Kwi |
| Name: |
sterile alpha motif domain containing 8; targeted mutation 2.1, Klaus Willecke |
| MGI ID: |
MGI:5707963 |
| Synonyms: |
SMSrflox |
| Gene: |
Samd8 Location: Chr14:21800599-21848794 bp, + strand Genetic Position: Chr14, 11.86 cM, cytoband B
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| Alliance: |
Samd8tm2.1Kwi page
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| Allele Type: |
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Targeted (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The modified gene contains a loxP site upstream of exon 5, a single frt site (left by FLP recombinase excision of a neomycin resistance cassette) followed by a second loxP site downstream of the 3' UTR, and a second copy of exon 5 followed in-frame by the EGFP coding sequence. Cre recombinase-mediated excision of the endogenous exon 5 through the 3' untranslated region will result in splicing of exon 4 to the downstream copy of exon 5, leading to production of a SAMD8/EGFP fusion protein lacking the catalytic domain encoded by exon 6.
(J:228568)
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| Original: |
J:228568 Bickert A, et al., Functional characterization of enzymes catalyzing ceramide phosphoethanolamine biosynthesis in mice. J Lipid Res. 2015 Apr;56(4):821-35 |
| All: |
1 reference(s) |
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Analysis Tools
