Gene Expression Data
Assay Details
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Assay
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Reference:
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J:148786
Barber M, et al., The role of Robo3 in the development of cortical interneurons. Cereb Cortex. 2009 Jul;19 Suppl 1:i22-31
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5752745
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Antibody:
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anti-Robo1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Results
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Specimen
2A/A':
embryonic day 13.5
(more  )
Note: Double labeled: green - Robo1; red - Reln (assay MGI:5752750).
Specimen
2A/A':
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Robo1; red - Reln (assay MGI:5752750).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex marginal layer |
Present |
Regionally restricted |
2A
,
2A'
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Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
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| TS21: cortical preplate |
Present |
Regionally restricted |
2A
,
2A'
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Expressed within the lower half of the preplate/marginal zone and below a single layer of strongly labeled reelin immunopositive cells, a marker of Cajal--Retzius cells.
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| TS21: cortical hem |
Present |
Regionally restricted |
2A
,
2A'
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Colocalization with reelin was also more prominent within the deeper layers of the cortical hem.
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Specimen
S1A:
embryonic day 11.5
(more )
Specimen
S1A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: telencephalon |
Present |
Regionally restricted |
S1A
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Expressed in the preplate layer, the lateral, medial and caudal ganglionic eminences.
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Specimen
S1D:
embryonic day 12.5
(more )
Specimen
S1D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cortical preplate |
Present |
Graded |
S1D
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In a decreasing mediolateral gradient.
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| TS20: lateral ganglionic eminence |
Present |
Regionally restricted |
S1D
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Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.
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| TS20: medial ganglionic eminence |
Present |
Regionally restricted |
S1D
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Robo1 and Robo3 showed similar patterns of expression both within the lateral and medial ganglionic eminences, and overlapped in regions where Robo1 protein was most robustly localized.
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Specimen
S1G:
embryonic day 13.5
(more )
Specimen
S1G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools