Plin2tm1.1(cre/ERT2)Mort
Targeted Allele Detail
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| Symbol: |
Plin2tm1.1(cre/ERT2)Mort |
| Name: |
perilipin 2; targeted mutation 1.1, Rory E Morty |
| MGI ID: |
MGI:5755034 |
| Gene: |
Plin2 Location: Chr4:86566623-86588297 bp, - strand Genetic Position: Chr4, 40.69 cM
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| Alliance: |
Plin2tm1.1(cre/ERT2)Mort page
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| Allele Type: |
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Targeted (Inducible, Recombinase, Reporter) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Plin2tm1.1(cre/ERT2)Mort expression driven by
1 gene
Knock-in expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Plin2 (MGI:87920) |
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Mutation details: A promoterless mCherry reporter gene, two T2A sequence and inducible cre recombinase fusion gene (cre/ERT2) were inserted into the exon 8 in-frame upstream of the endogenous Plin2 stop codon by homologous recombination using a targeting vector encoding 6 kb 50 and 3.6 kb 30 homologous sequences. Two T2A peptide sequences separate Plin2 and mCherry and CreERT2 from each other. The targeting vector also contained a diptheria toxin A cassette for negative and a FRT-flanked PGK-neomycin cassette downstream of the mCherry-CreERT2 cassette for positive selection. Chimeric males were backcrossed onto C57BL/6J background to achieve germline transmission and bred with a mouse expressing Flp to remove the FRT-PGK-neomycin selection cassette.
(J:229959, J:250476)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Plin2
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Plin2 Mutation: |
38 strains or lines available
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| Original: |
J:229959 Morty RE, Direct Data Submission for a Plin2 allele. MGI Direct Data Submission. 2016; |
| All: |
3 reference(s) |
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Analysis Tools
