Col1a1<tm17(tetO-GFP,-cas9*)Slowe> Targeted Allele Detail MGI Mouse (MGI:5755839)

Col1a1^{tm17(tetO-GFP,-cas9*)Slowe}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm17(tetO-GFP,-cas9*)Slowe}
Name:	collagen, type I, alpha 1; targeted mutation 17, Scott Lowe
MGI ID:	MGI:5755839
Synonyms:	Col1a1^{tm17(RNU6-gRNA:Apc,tetO-GFP,-cas9*)Slowe}
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm17(tetO-GFP,-cas9*)Slowe} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:222595
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:		Targeted (Endonuclease, Inducible, Reporter)
Inducer:		doxycycline/tetracycline
Mutation:		Insertion
		Mutation details: RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a single guide RNA targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence and an tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease cas9 (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:222595 Dow LE, et al., Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol. 2015 Apr;33(4):390-4
All:	1 reference(s)

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