Probe:	TGF-beta-3 cDNA3
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were reduced to an average size of 100-150 nucleotides by limited alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	1A/B	Expression is in the meninges and choroid plexus.
TS19: axial skeleton	Present	Regionally restricted	1A/B	Expression is in the intervertebral disks.
TS19: liver	Present	Regionally restricted	1A/B	Expression is in the liver capsule.
TS19: extraembryonic component	Present	Regionally restricted	1A/B	Expression is in the extraembryonic membranes.
TS19: future spinal cord	Present	Regionally restricted	1A/B	Expression is in the meninges.
TS19: body wall	Present		1A/B
TS19: heart	Present		1A/B
TS19: lung	Present		1A/B
TS19: trachea	Present		1A/B

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Present	Regionally restricted	2A/E	Expression is in the mesenchymal cells surrounding the notochord and in a small region of condensing mesenchyme ventral to the notochord.
TS19: notochord	Absent		2A/E

Structure	Level	Pattern	Image	Note
TS19: embryo mesenchyme	Present	Regionally restricted	2B/F	Expression is in the mesenchymal cells surrounding the notochord and in a small region of condensing mesenchyme ventral to the notochord.
TS19: notochord	Absent		2B/F

Structure	Level	Pattern	Image	Note
TS19: axial skeleton	Present	Regionally restricted	2C/G	Expression is in the condensations of perichordal mesenchyme between each vertebral body (intervertebral discs).

Structure	Level	Pattern	Image	Note
TS21: intervertebral disc	Strong		2D/H

Structure	Level	Pattern	Image	Note
TS24: hindlimb skeleton	Weak	Regionally restricted	3C/F	Expression is in the perichondrial region surrounding the mature cartilage.

Structure	Level	Pattern	Image	Note
TS23: maxilla	Present	Regionally restricted	4D	Expression is in osteoblasts.
TS23: upper jaw mesenchyme	Weak	Regionally restricted	4D	Expression is in the mesenchyme surrounding the bone.

Structure	Level	Pattern	Image	Note
TS21: tooth mesenchyme	Present	Regionally restricted	5C/F	Expression is in the non-condensed mesenchyme adjacent and surrounding the condensed mesenchyme.
TS21: tooth epithelium	Absent		5C/F

Structure	Level	Pattern	Image	Note
TS24: dental papilla	Present	Regionally restricted	5I/L	Expression is in the odontoblast layer and is low in the pulp.
TS24: enamel organ	Absent		5I/L

Structure	Level	Pattern	Image	Note
TS19: body wall	Very strong		6A/B
TS19: liver	Very strong	Regionally restricted	6A/B	Expression is in the capsule.
TS19: atrioventricular canal	Strong	Regionally restricted	6A/B	Expression is in the atrioventricular cushions.
TS19: heart ventricle	Absent		6A/B

Structure	Level	Pattern	Image	Note
TS23: trachea mesenchyme	Present	Regionally restricted	6C/D	Expression is in the submucosa.
TS23: trachea cartilaginous ring	Present	Regionally restricted	6C/D	Expression is in the perichondrium.
TS23: cartilage	Present	Regionally restricted	6C/D	Expression is in the cartilage adjacent to the trachea.
TS23: trachea epithelium	Absent		6C/D

Structure	Level	Pattern	Image	Note
TS19: liver	Present	Regionally restricted	6E/F	Expression is in the capsule.
TS19: liver parenchyma	Absent		6E/F

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Very strong	Regionally restricted	7A/B	Expression is in the medial edge of the secondary palate.

Structure	Level	Pattern	Image	Note
TS23: pleural component	Present	Regionally restricted	7C/D	Expression is in the pleura surrounding the lung.
TS23: bronchus epithelium	Present	Regionally restricted	7C/D	Expression is in the proximal bronchial epithelium, absent in the distal epithelium.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	7E/F	Expression is in the ependymal epithelium at the base of the choroid plexus and is low in the choroid plexus.

Structure	Level	Pattern	Image	Note
TS21: vomeronasal organ mesenchyme	Weak	Diffuse	8E/F	Expression is surrounding the neuroepithelium.
TS21: nasal septum	Present	Regionally restricted	8E/F	Expression is in the perichondrium of the nasal cartilage.
TS21: vomeronasal organ epithelium	Absent		8E/F

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Regionally restricted	9B/C , 9A	Expression is in the intermediate zone, absent in the ventricular layer.
TS22: brain meninges	Present		9A , 9B/C

Structure	Level	Pattern	Image	Note
TS22: brain meninges	Strong		9D , 9E/F
TS22: main olfactory bulb	Strong	Regionally restricted	9E/F , 9D	Expression is in the intermediate zone, absent in the ventricular layer.

Structure	Level	Pattern	Image	Note
TS23: main olfactory bulb	Present	Regionally restricted	9H/I , 9G	Expression is in the mitral cell layer on the rostral periphery of the bulb.
TS23: nasal septum cartilage	Weak	Regionally restricted	9H/I , 9G	Expression is in the perichondrium.
TS23: olfactory epithelium	Absent		9G , 9H/I

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb mitral cell layer	Strong		9K/L , 9J
TS24: telencephalon meninges	Strong		9K/L , 9J