Nkx2-2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:160812 Ribes V, et al., Distinct Sonic Hedgehog signaling dynamics specify floor plate and ventral neuronal progenitors in the vertebrate neural tube. Genes Dev. 2010 Jun 1;24(11):1186-200
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5766913
Gene symbol:	Nkx2-2
Gene name:	NK2 homeobox 2

Antibody:

74.5A5

Results

1B ©

Specimen 1B: embryonic day 8.0 (more

)
Note: 7 somites. Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS12: neural tube lateral wall	Present	Regionally restricted	1B	Expression is in p3 progenitors in the ventral midline.

1C ©

Specimen 1C: embryonic day 8.0 (more

)
Note: 7 somites. Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS12: neural tube lateral wall	Present	Regionally restricted	1C	Expression is in p3 progenitors in the ventral midline.

1D ©

1D' ©

Specimen 1D: embryonic day 8.5 (more

)
Note: 10 somites. Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS13: neural tube lateral wall	Present	Regionally restricted	1D' , 1D	Expression is in p3 progenitors in the ventral midline.

1E ©

1E' ©

1E'' ©

Specimen 1E: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	1E , 1E'' , 1E'	Expression is in p3 progenitors in the ventral midline.

1F ©

Specimen 1F: embryonic day 10.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1F	Expression is in p3 progenitors in the ventral midline.

1G ©

1G' ©

Specimen 1G: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	1G , 1G'	Expression is in p3 progenitors in the ventral midline.

1H ©

Specimen 1H: embryonic day 10.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	1H	Expression is in p3 progenitors in the ventral midline.

2H inset ©

Specimen 2H inset: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	2H inset	Expression is in p3 progenitors in the ventral midline.

2I inset ©

Specimen 2I inset: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	2I inset	Expression is in p3 progenitors in the ventral midline.

S1_A left ©

S1_A right ©

Specimen S1 A: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S1_A right , S1_A left	Expression is in p3 progenitors in the ventral midline.

S1_B left ©

S1_B right ©

Specimen S1 B: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S1_B right , S1_B left	Expression is in p3 progenitors in the ventral midline.

S1_C left ©

S1_C right ©

Specimen S1 C: embryonic day 9.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S1_C left , S1_C right	Expression is in p3 progenitors in the ventral midline.

S1_D left ©

S1_D right ©

Specimen S1 D: embryonic day 10.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	S1_D right , S1_D left	Expression is in p3 progenitors in the ventral midline.

S1_E left ©

S1_E right ©

Specimen S1 E: embryonic day 10.5 (more

)
Note: Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	S1_E right , S1_E left	Expression is in p3 progenitors in the ventral midline.

S1_F left ©

S1_F right ©

Specimen S1 F: embryonic day 10.5 (more

)
Note: Lumbar level. Triple labeled: green - Nkx2-2; red - Foxa2 (assay MGI:5766916); blue - Arx (assay MGI:5766912).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	S1_F left , S1_F right	Expression is in p3 progenitors in the ventral midline.

S1_H ©

Specimen S1 H: embryonic day 10.5; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor, Olig2^tm1(cre)Tmj/Olig2⁺ (more

)
Note: Conditional mutant. Lumbar level.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	S1_H	Expression is in p3 progenitors in the ventral midline.

S1_H' ©

Specimen S1 H': embryonic day 10.5; Gt(ROSA)26Sor^tm1Sor/Gt(ROSA)26Sor^tm1Sor, Olig2^tm1(cre)Tmj/Olig2⁺ (more

)
Note: Conditional mutant. Cervical level.

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	S1_H'	Expression is in p3 progenitors in the ventral midline.

S4_F ©

Specimen S4 F: embryonic day 9.5; Ptch1^tm1Mps/Ptch1⁺ (more

)
Note: Forelimb level. Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S4_F	Expression is in p3 progenitors in the ventral midline.

S4_G ©

Specimen S4 G: embryonic day 9.5; Ptch1^tm1Mps/Ptch1⁺ (more

)
Note: Lumbar level. Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S4_G	Expression is in p3 progenitors in the ventral midline.

S4_H ©

Specimen S4 H: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Forelimb level. Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S4_H	Expression is in p3 progenitors in the ventral midline.

S4_I ©

Specimen S4 I: embryonic day 9.5; Ptch1^tm1Mps/Ptch1^tm1Mps (more

)
Note: Triple labeled: green - Nkx2-2; red - Arx (assay MGI:5766912); blue - Olig2 (assay MGI:5766915).

Structure	Level	Pattern	Image	Note
TS15: neural tube lateral wall	Present	Regionally restricted	S4_I	Expression is in p3 progenitors in the ventral midline.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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