Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:14610
Curto KA, et al., Immunocytochemical localization of gamma-glutamyltranspeptidase during fetal development of mouse kidney. J Histochem Cytochem. 1988 Feb;36(2):159-66
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5770227
|
|
Gene symbol:
|
Ggt1
|
|
Gene name:
|
gamma-glutamyltransferase 1
|
|
Antibody:
|
anti-gammaGT
|
|
Detection system:
|
Secondary antibody coupled to horseradish peroxidase
|
|
Assay notes:
|
Fixative: 2% formaldehyde; 2.5% glutaraldehyde.
|
|
|
Results
|
Specimen
4a/b:
embryonic day 13.0
(more  )
Note: Embedded in methacrylate.
Specimen
4a/b:
(close  )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: renal vesicle |
Present |
Regionally restricted |
4a/b
|
Expression was localized to the apical surface of the vesicles.
|
Specimen
5a/b:
embryonic day 13.0
(more )
Note: Embedded in methacrylate.
Specimen
5a/b:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 13.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: s-shaped body |
Present |
Regionally restricted |
5a/b
|
Expression was localized to the apical surface of the segment that is the precursor of the proximal tubule. The layer of cells that will form Bowman's capsule was not labeled.
|
| TS21: proximal segment of s-shaped body |
Present |
Regionally restricted |
5a/b
|
Expression was localized to the apical surface.
|
Specimen
6a:
embryonic day 15.0
(more )
Note: Embedded in methacrylate.
Specimen
6a:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 15.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
Specimen
6b:
embryonic day 17.0
(more )
Note: Embedded in methacrylate.
Specimen
6b:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cortical early proximal tubule |
Present |
Regionally restricted |
6b
|
Expression was localized to the apical surface of the proximal tubular segments. Slight basolateral surface immunoreactivity was evident.
|
Specimen
6c:
embryonic day 17.0
(more )
Note: Embedded in methacrylate.
Specimen
6c:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cortical early proximal tubule |
Present |
Regionally restricted |
6c
|
Expression was localized to the apical surface of the proximal tubular segments. Slight basolateral surface immunoreactivity was evident.
|
Specimen
not shown 21 days:
postnatal day 21
(more )
Note: Embedded in methacrylate.
Specimen
not shown 21 days:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
postnatal day 21
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal proximal tubule |
Present |
Regionally restricted |
|
Expression was localized to the apical surface of the proximal tubules in the fully differentiated area of the renal cortex. Slight basolateral surface immunoreactivity was evident.
|
Specimen
not shown adult:
postnatal adult
(more )
Note: Embedded in methacrylate.
Specimen
not shown adult:
(close )
|
Genetic Background:
|
Swiss Webster
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Resin/plastic
|
|
Note:
|
Embedded in methacrylate.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: renal proximal tubule |
Present |
Regionally restricted |
|
Expression was localized to the apical surface of the proximal tubules in the fully differentiated area of the renal cortex. Slight basolateral surface immunoreactivity was evident.
|
|
Analysis Tools