Probe:	Tgfb3 probe7
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: spongiotrophoblast	Strong		2C	Strong expression exclusively in the spongiotrophoblast.

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent		3C	Only non-specific background signals are visible.

Structure	Level	Pattern	Image	Note
TS20: gut mesentery	Moderate	Regionally restricted	4C	Moderate level of expression in mesenteric epithelium surrounding the fetal liver, and in mesenteric mesoderm of the gut.
TS20: vertebral cartilage condensation	Strong	Regionally restricted	4C	High levels of expression in primordia of vertebral column. Expression in all prevertebral segments.
TS20: vertebral pre-cartilage condensation	Strong	Regionally restricted	4C	High levels of expression in primordia of vertebral column. Expression in all prevertebral segments.
TS20: sclerotome	Strong	Regionally restricted	4C	Strongest expression in caudal sclerotomic halves.
TS20: trachea mesenchyme	Present		4C	Expression predominantly in tracheal mesenchyme.
TS20: gut mesenchyme	Moderate		4C	Moderate levels of expression in both submucosal mesenchyme and mesenteric mesoderm.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Regionally restricted	5C	Expression in the perichondrium of all cartilaginous elements of the axial skeleton; levels of expression varied.
TS22: vertebral cartilage condensation	Strong	Regionally restricted	5C	Strong expression in undifferentiated mesenchymal cell layers adjacent to ossification centers.
TS22: bronchiole epithelium	Present		5C	Expression in endodermal epithelial cells of growing bronchioles.
TS22: visceral pleura	Present		5C	Expression in mesodermal epithelial cells giving rise to visceral pleura.
TS22: gut mesentery	Present		5C	Expression confined to mesentery
TS22: metanephros	Absent		5C
TS22: vibrissa root sheath	Strong		5C	High levels of expression in ectodermal epithelium of external root sheath.

Structure	Level	Pattern	Image	Note
TS24: rib	Weak		6B	Only very low levels of expression in vertebra and ribs.
TS24: vertebra	Weak		6B	Only very low levels of expression in vertebra and ribs.
TS24: perichondrium	Present	Regionally restricted	6B	Expression in the perichondrium of the limb skeleton.
TS24: limb bone	Strong	Regionally restricted	6B	Strong expression in undifferentiated mesenchymal cell layers adjacent to ossification centers.
TS24: lung	Absent		6B
TS24: gut mesentery	Present		6B

Structure	Level	Pattern	Image	Note
TS24: upper jaw mesenchyme	Strong	Regionally restricted	8C	Strong expression in undifferentiated mesenchymal cells surrounding upper jaw bones.
TS24: ethmoid bone	Strong	Regionally restricted	8C	Strong expression in periosteal layers of the ethmoid cartilage.

Structure	Level	Pattern	Image	Note
TS17: mesenchyme derived from neural crest	Absent
TS17: yolk sac	Absent

Structure	Level	Pattern	Image	Note
TS20: cochlea epithelium	Absent
TS20: thymus primordium	Absent

Structure	Level	Pattern	Image	Note
TS22: intervertebral disc	Strong			Within the vertebral column, strongest expression in the intervertebral discs.
TS22: cochlea epithelium	Absent

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Absent
TS24: dermis	Absent
TS24: vibrissa inner root sheath	Present			Expression in both inner and outer root sheath of whisker follicles.
TS24: vibrissa outer root sheath	Present			Expression in both inner and outer root sheath of whisker follicles.
TS24: vibrissa root sheath matrix	Absent			No expression in connective tissue sheath of the hair follicle.