Col1a1<tm1(tetO-cas9)Sho> Targeted Allele Detail MGI Mouse (MGI:5781096)

Col1a1^{tm1(tetO-cas9)Sho}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm1(tetO-cas9)Sho}
Name:	collagen, type I, alpha 1; targeted mutation 1, Stuart Orkin
MGI ID:	MGI:5781096
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm1(tetO-cas9)Sho} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:		Targeted (Endonuclease, Inducible)
Inducer:		doxycycline/tetracycline
Mutation:		Insertion
		Mutation details: KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sor allele were re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette downstream of the Col1a1 locus. Correctly re-targeted ES cells were selected and the FRT site-flanked PGK-Neo downstream of the Col1a1 locus was replaced by electroporating both a "flip-in plasmid" containing a PGK promoter, ATG start codon, FRT site, a splice acceptor-double polyA sequence (SA-2xpA), tetO (tetracycline responsive element) with a CMV minimal promoter, and SV40 polyA signal, upstream of the hCas9 (human codon optimized Cas9 nuclease) sequence, and a FLPe recombinase expressing vector to facilitate tetO-hCAS9 recombination into the 3' UTR of the Col1a1 locus. (J:101977)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	163 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae}. This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	4 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24