Tg(Dmp1-cre/ERT2)0022Pdp
Transgene Detail
|
|
| Symbol: |
Tg(Dmp1-cre/ERT2)0022Pdp |
| Name: |
transgene insertion 0022, Paola Divieti Pajevic |
| MGI ID: |
MGI:5792965 |
| Synonyms: |
10kb-Dmp1-Cre-ERT2, Tg(Dmp1-cre/ERT2)1Pdp |
| Transgene: |
Tg(Dmp1-cre/ERT2)0022Pdp Location: unknown
|
| Alliance: |
Tg(Dmp1-cre/ERT2)0022Pdp page
|
|
|
|
| Transgene Type: |
|
Transgenic (Inducible, Recombinase) |
| Inducer: |
|
tamoxifen |
| Mutation: |
|
Insertion
|
| |
|
Tg(Dmp1-cre/ERT2)0022Pdp expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| mouse |
Dmp1 (MGI:94910) |
|
|
| |
|
Mutation details: A fragment (-9624 - +4439) of the mouse Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of initial non-coding region of exon 2 was cloned into a vector in front of the cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. The two transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts.
(J:170229)
|
|
|
|
Activity: |
 Tissue activity of this recombinase allele
|
|
Driver:
|
Dmp1
(mouse)
|
| |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
| Original: |
J:170229 Powell WF Jr, et al., Targeted ablation of the PTH/PTHrP receptor in osteocytes impairs bone structure and homeostatic calcemic responses. J Endocrinol. 2011 Apr;209(1):21-32 |
| All: |
12 reference(s) |
|
Analysis Tools
